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Influence of CaCl2 on Penicillium digitatum, grapefruit peel tissue, and biocontrol activity of Pichia guilliermondii
Year:
1997
Source of publication :
Phytopathology
Authors :
Chalutz, Edo
;
.
Cohen, Lydia
;
.
Droby, Samir
;
.
Weiss, Batia
;
.
Volume :
87
Co-Authors:
Droby, S., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wisniewski, M.E., USDA-ARS, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Cohen, L., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Weiss, B., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Touitou, D., Department of Bacteriology, Hebrew University of Jerusalem, Hadassa Medical School, Jerusalem, Israel
Eilam, Y., Department of Bacteriology, Hebrew University of Jerusalem, Hadassa Medical School, Jerusalem, Israel
Chalutz, E., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
310
To page:
315
(
Total pages:
6
)
Abstract:
Interactions between CaCl2, grapefruit peel tissue, Penicillium digitatum, and the yeast antagonist Pichia guilliermondii strain US-7 were investigated. Application of 68 or 136 mM CaCl2 to grapefruit surface wounds reduced the incidence of green mold caused by Penicillium digitatum by 43 to 52%. In laboratory tests, a cell suspension (107 cells/ml) of Pichia guilliermondii containing either 68 or 136 mM CaCl2 reduced the incidence of green mold from 27 to 3%. In large scale tests, dip application of 136 mM CaCl2 with US-7 (107 cells/ml) significantly decreased the number of wounds infected by Penicillium digitatum. CaCl2, with or without yeast cells, stimulated ethylene production in grapefruit tissue. Increasing concentrations of CaCl2 resulted in decreased spore germination and germ tube elongation of Penicillium digitatum. Pectinolytic activity of crude enzyme preparations of Penicillium digitatum was also inhibited by the presence of increasing concentrations of CaCl2 US-7 exhibited a strong ability to maintain cytosolic Ca2+ homeostasis at levels that did not exceed 1.4 μM when exposed to 150 mM CaCl2. On the other hand, strain 114 of Debaryomyces hansenii, which failed to give any protection against infection by Penicillium digitatum, showed reduced capacity to maintain Ca2+ homeostasis. The effect of calcium in reducing infection of grapefruit wounds by Penicillium digitatum could be due to direct effects on host tissue (making cell walls more resistant to enzymatic degradation) or the pathogen (interfering with spore germination, growth, and inhibition of fungal pectinolytic enzymes). Alternatively, the ability of US-7 to maintain calcium homeostasis may allow it to grow or assist in its competitive ability in a microenvironment that, because of high levels of calcium ions, is inhibitory to growth of the green mold pathogen.
Note:
Related Files :
Penicillium digitatum
Pichia guilliermondii
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Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30636
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
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Influence of CaCl2 on Penicillium digitatum, grapefruit peel tissue, and biocontrol activity of Pichia guilliermondii
87
Droby, S., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wisniewski, M.E., USDA-ARS, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Cohen, L., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Weiss, B., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Touitou, D., Department of Bacteriology, Hebrew University of Jerusalem, Hadassa Medical School, Jerusalem, Israel
Eilam, Y., Department of Bacteriology, Hebrew University of Jerusalem, Hadassa Medical School, Jerusalem, Israel
Chalutz, E., Department of Postharvest Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Influence of CaCl2 on Penicillium digitatum, grapefruit peel tissue, and biocontrol activity of Pichia guilliermondii
Interactions between CaCl2, grapefruit peel tissue, Penicillium digitatum, and the yeast antagonist Pichia guilliermondii strain US-7 were investigated. Application of 68 or 136 mM CaCl2 to grapefruit surface wounds reduced the incidence of green mold caused by Penicillium digitatum by 43 to 52%. In laboratory tests, a cell suspension (107 cells/ml) of Pichia guilliermondii containing either 68 or 136 mM CaCl2 reduced the incidence of green mold from 27 to 3%. In large scale tests, dip application of 136 mM CaCl2 with US-7 (107 cells/ml) significantly decreased the number of wounds infected by Penicillium digitatum. CaCl2, with or without yeast cells, stimulated ethylene production in grapefruit tissue. Increasing concentrations of CaCl2 resulted in decreased spore germination and germ tube elongation of Penicillium digitatum. Pectinolytic activity of crude enzyme preparations of Penicillium digitatum was also inhibited by the presence of increasing concentrations of CaCl2 US-7 exhibited a strong ability to maintain cytosolic Ca2+ homeostasis at levels that did not exceed 1.4 μM when exposed to 150 mM CaCl2. On the other hand, strain 114 of Debaryomyces hansenii, which failed to give any protection against infection by Penicillium digitatum, showed reduced capacity to maintain Ca2+ homeostasis. The effect of calcium in reducing infection of grapefruit wounds by Penicillium digitatum could be due to direct effects on host tissue (making cell walls more resistant to enzymatic degradation) or the pathogen (interfering with spore germination, growth, and inhibition of fungal pectinolytic enzymes). Alternatively, the ability of US-7 to maintain calcium homeostasis may allow it to grow or assist in its competitive ability in a microenvironment that, because of high levels of calcium ions, is inhibitory to growth of the green mold pathogen.
Scientific Publication
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