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Ammonium secretion by Colletotrichum coccodes activates host NADPH oxidase activity enhancing host cell death and fungal virulence in tomato fruits
Year:
2009
Authors :
Alkan, Noam
;
.
Prusky, Dov
;
.
Volume :
22
Co-Authors:
Alkan, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Davydov, O., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Sagi, M., Department of Dryland Biotechnologies, Sede Boqer Campus, Ben-Gurion University, Midreshet Ben-Gurion 84990, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
1484
To page:
1491
(
Total pages:
8
)
Abstract:
Colletotrichum pathogens of fruit and leaves are known ammonium secretors. Here, we show that Colletotrichum coccodes virulence, as measured by tomato (Solanum ly-copersicum cv. Motelle) fruit tissue necrosis, correlates with the amount of ammonium secreted. Ammonium application to fruit tissue induced hydrogen peroxide (H2O2) accumulation. To examine whether the tomato NADPH oxidase, SlRBOH, is a source for the ammonium-induced H 2O2, wild-type and antisense lines abrogated for SlRBOH (SlRBOH-AS) were examined. Wild-type lines produced 7.5-fold more reactive oxygen species when exposed to exogenous ammonium than did SlRBOH-AS lines. C. coccodes colonization of wild-type tomato lines resulted in higher H 2O2 production and faster fungal growth rate compared with colonization in the SlRBOH-AS mutant, although the amount of ammonium secreted by the fungi was similar in both cases. Enhanced ion leakage and cell death of fruit tissue were correlated with H2O2 accumulation, and treatment with the reactive oxygen scavenger N-acetyl-L-cysteine decreased H2O2 production, ion leakage, and cell death. Importantly, the activation of reactive oxygen species production by ammonium was positively affected by an extracellular pH increase from 4 to 9, implying that ammonium exerts its control via membrane penetration. Our results show that C. coccodes activates host reactive oxygen species and H2O2 production through ammonium secretion. The resultant enhancement in host tissue decay is an important step in the activation of the necrotrophic process needed for colonization. © 2009 The American Phytopathological Society.
Note:
Related Files :
Colletotrichum
fungi
metabolism
Microbiology
pH
Plant Disease
Solanum
Show More
Related Content
More details
DOI :
10.1094/MPMI-22-12-1484
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30647
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
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Scientific Publication
Ammonium secretion by Colletotrichum coccodes activates host NADPH oxidase activity enhancing host cell death and fungal virulence in tomato fruits
22
Alkan, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Davydov, O., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Sagi, M., Department of Dryland Biotechnologies, Sede Boqer Campus, Ben-Gurion University, Midreshet Ben-Gurion 84990, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ammonium secretion by Colletotrichum coccodes activates host NADPH oxidase activity enhancing host cell death and fungal virulence in tomato fruits
Colletotrichum pathogens of fruit and leaves are known ammonium secretors. Here, we show that Colletotrichum coccodes virulence, as measured by tomato (Solanum ly-copersicum cv. Motelle) fruit tissue necrosis, correlates with the amount of ammonium secreted. Ammonium application to fruit tissue induced hydrogen peroxide (H2O2) accumulation. To examine whether the tomato NADPH oxidase, SlRBOH, is a source for the ammonium-induced H 2O2, wild-type and antisense lines abrogated for SlRBOH (SlRBOH-AS) were examined. Wild-type lines produced 7.5-fold more reactive oxygen species when exposed to exogenous ammonium than did SlRBOH-AS lines. C. coccodes colonization of wild-type tomato lines resulted in higher H 2O2 production and faster fungal growth rate compared with colonization in the SlRBOH-AS mutant, although the amount of ammonium secreted by the fungi was similar in both cases. Enhanced ion leakage and cell death of fruit tissue were correlated with H2O2 accumulation, and treatment with the reactive oxygen scavenger N-acetyl-L-cysteine decreased H2O2 production, ion leakage, and cell death. Importantly, the activation of reactive oxygen species production by ammonium was positively affected by an extracellular pH increase from 4 to 9, implying that ammonium exerts its control via membrane penetration. Our results show that C. coccodes activates host reactive oxygen species and H2O2 production through ammonium secretion. The resultant enhancement in host tissue decay is an important step in the activation of the necrotrophic process needed for colonization. © 2009 The American Phytopathological Society.
Scientific Publication
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