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Molecular changes occurring during acquisition of abscission competence following auxin depletion in Mirabilis jalapa
Year:
2006
Source of publication :
Plant physiology (source)
Authors :
Halaly, Vita
;
.
Meir, Shimon
;
.
Volume :
141
Co-Authors:
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Hunter, D.A., New Zealand Institute for Crop and Food Research Limited, Private Bag 11600, Palmerston North, New Zealand
Chen, J.-C., Department of Plant Sciences, University of California, Davis, CA 95616, United States
Halaly, V., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Reid, M.S., Department of Plant Sciences, University of California, Davis, CA 95616, United States
Facilitators :
From page:
1604
To page:
1616
(
Total pages:
13
)
Abstract:
To understand how auxin regulates sensitivity of abscission zone (AZ) tissues to ethylene, we used a polymerase chain reaction-based subtractive approach to identify gene transcripts in Mirabilis jalapa AZs that changed in abundance during the time the zones became competent to abscise in response to exogenous ethylene. Transcript expression was then examined in leaf and stem AZs over the period they became ethylene competent following indole-3-acetic acid (IAA) depletion either by leaf deblading, treatment with the IAA transport inhibitor naphthylphthalamic acid, or cutting the stem above a node (decapitation). Transcripts down-regulated by deblading/decapitation included Mj-Aux/IAA1 and Mj-Aux/IAA2, encoding Aux/IAA proteins, and three other transcripts snowing highest identity to a polygalacturonase inhibitor protein, a β-expansin, and a β-tubulin. Application of IAA to the cut end of petioles or stumps inhibited abscission, and prevented the decline in the levels of transcripts in both AZs. Transcripts up-regulated in the AZ following deblading/decapitation or treatment with naphthylphthalamic acid were isolated from plants pretreated with 1-methylcyclopropene before deblading to help select against ethylene-induced genes. Some of the up-regulated transcripts showed identity to proteins associated with ethylene or stress responses, while others did not show homology to known sequences. Sucrose infiltration of stem stumps enhanced abscission following ethylene treatment and also enhanced the induction of some of the up-regulated genes. Our results demonstrate a correlation between acquisition of competence to respond to ethylene in both leaf and stem AZs, and decline in abundance of auxin regulatory gene transcripts. © 2006 American Society of Plant Biologists.
Note:
Related Files :
ethylene
Genetics
molecular genetics
Plants
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More details
DOI :
10.1104/pp.106.079277
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30656
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
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Scientific Publication
Molecular changes occurring during acquisition of abscission competence following auxin depletion in Mirabilis jalapa
141
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Hunter, D.A., New Zealand Institute for Crop and Food Research Limited, Private Bag 11600, Palmerston North, New Zealand
Chen, J.-C., Department of Plant Sciences, University of California, Davis, CA 95616, United States
Halaly, V., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Reid, M.S., Department of Plant Sciences, University of California, Davis, CA 95616, United States
Molecular changes occurring during acquisition of abscission competence following auxin depletion in Mirabilis jalapa
To understand how auxin regulates sensitivity of abscission zone (AZ) tissues to ethylene, we used a polymerase chain reaction-based subtractive approach to identify gene transcripts in Mirabilis jalapa AZs that changed in abundance during the time the zones became competent to abscise in response to exogenous ethylene. Transcript expression was then examined in leaf and stem AZs over the period they became ethylene competent following indole-3-acetic acid (IAA) depletion either by leaf deblading, treatment with the IAA transport inhibitor naphthylphthalamic acid, or cutting the stem above a node (decapitation). Transcripts down-regulated by deblading/decapitation included Mj-Aux/IAA1 and Mj-Aux/IAA2, encoding Aux/IAA proteins, and three other transcripts snowing highest identity to a polygalacturonase inhibitor protein, a β-expansin, and a β-tubulin. Application of IAA to the cut end of petioles or stumps inhibited abscission, and prevented the decline in the levels of transcripts in both AZs. Transcripts up-regulated in the AZ following deblading/decapitation or treatment with naphthylphthalamic acid were isolated from plants pretreated with 1-methylcyclopropene before deblading to help select against ethylene-induced genes. Some of the up-regulated transcripts showed identity to proteins associated with ethylene or stress responses, while others did not show homology to known sequences. Sucrose infiltration of stem stumps enhanced abscission following ethylene treatment and also enhanced the induction of some of the up-regulated genes. Our results demonstrate a correlation between acquisition of competence to respond to ethylene in both leaf and stem AZs, and decline in abundance of auxin regulatory gene transcripts. © 2006 American Society of Plant Biologists.
Scientific Publication
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