נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Genetic diversity and a PCR-based method for xanthomonas axonopodis detection in passion fruit
Year:
2011
Source of publication :
Phytopathology
Authors :
Weiss, Batia
;
.
Volume :
101
Co-Authors:
Munhoz, C.F., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Weiss, B., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil, Dow AgroSciences no Brasil, Rodovia Anhanguera Km 296, Cravinhos, Brazil
Hanai, L.R., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Zucchi, M.I., Instituto Agronǒmico, P.O. Box 28, 13012-970 Campinas, Brazil
Fungaro, M.H.P., Universidade Estadual de Londrina, P.O. Box 6001, 86051-970 Londrina, Brazil
Oliveira, A.L.M., Universidade Estadual de Londrina, P.O. Box 6001, 86051-970 Londrina, Brazil
Monteiro-Vitorello, C.B., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Vieira, M.L.C., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Facilitators :
From page:
416
To page:
424
(
Total pages:
9
)
Abstract:
Xanthomonas axonopodis pv. passiflorae causes bacterial spot in passion fruit. It attacks the purple and yellow passion fruit as well as the sweet passion fruit. The diversity of 87 isolates of pv. passiflorae collected from across 22 fruit orchards in Brazil was evaluated using molecular profiles and statistical procedures, including an unweighted pair-group method with arithmetical averages-based dendrogram, analysis of molecular variance (AMOVA), and an assigning test that provides information on genetic structure at the population level. Isolates from another eight pathovars were included in the molecular analyses and all were shown to have a distinct repetitive sequence-based polymerase chain reaction profile. Amplified fragment length polymorphism technique revealed considerable diversity among isolates of pv. passiflorae, and AMOVA showed that most of the variance (49.4%) was due to differences between localities. Cluster analysis revealed that most genotypic clusters were homogeneous and that variance was associated primarily with geographic origin. The disease adversely affects fruit production and may kill infected plants. A method for rapid diagnosis of the pathogen, even before the disease symptoms become evident, has value for producers. Here, a set of primers (Xapas) was designed by exploiting a singlenucleotide polymorphism between the sequences of the intergenic 16S- 23S rRNA spacer region of the pathovars. Xapas was shown to effectively detect all pv. passiflorae isolates and is recommended for disease diagnosis in passion fruit orchards. © 2011 The American Phytopathological Society.
Note:
Related Files :
Genetics
Passiflora
Show More
Related Content
More details
DOI :
10.1094/PHYTO-06-10-0169
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30666
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
You may also be interested in
Scientific Publication
Genetic diversity and a PCR-based method for xanthomonas axonopodis detection in passion fruit
101
Munhoz, C.F., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Weiss, B., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil, Dow AgroSciences no Brasil, Rodovia Anhanguera Km 296, Cravinhos, Brazil
Hanai, L.R., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Zucchi, M.I., Instituto Agronǒmico, P.O. Box 28, 13012-970 Campinas, Brazil
Fungaro, M.H.P., Universidade Estadual de Londrina, P.O. Box 6001, 86051-970 Londrina, Brazil
Oliveira, A.L.M., Universidade Estadual de Londrina, P.O. Box 6001, 86051-970 Londrina, Brazil
Monteiro-Vitorello, C.B., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Vieira, M.L.C., Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, P.O. Box 83, 13400-970 Piracicaba, Brazil
Genetic diversity and a PCR-based method for xanthomonas axonopodis detection in passion fruit
Xanthomonas axonopodis pv. passiflorae causes bacterial spot in passion fruit. It attacks the purple and yellow passion fruit as well as the sweet passion fruit. The diversity of 87 isolates of pv. passiflorae collected from across 22 fruit orchards in Brazil was evaluated using molecular profiles and statistical procedures, including an unweighted pair-group method with arithmetical averages-based dendrogram, analysis of molecular variance (AMOVA), and an assigning test that provides information on genetic structure at the population level. Isolates from another eight pathovars were included in the molecular analyses and all were shown to have a distinct repetitive sequence-based polymerase chain reaction profile. Amplified fragment length polymorphism technique revealed considerable diversity among isolates of pv. passiflorae, and AMOVA showed that most of the variance (49.4%) was due to differences between localities. Cluster analysis revealed that most genotypic clusters were homogeneous and that variance was associated primarily with geographic origin. The disease adversely affects fruit production and may kill infected plants. A method for rapid diagnosis of the pathogen, even before the disease symptoms become evident, has value for producers. Here, a set of primers (Xapas) was designed by exploiting a singlenucleotide polymorphism between the sequences of the intergenic 16S- 23S rRNA spacer region of the pathovars. Xapas was shown to effectively detect all pv. passiflorae isolates and is recommended for disease diagnosis in passion fruit orchards. © 2011 The American Phytopathological Society.
Scientific Publication
You may also be interested in