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Novel cis-acting elements in petunia Cab gene promoters
Year:
1989
Source of publication :
MGG Molecular & General Genetics
Authors :
Gidoni, David
;
.
Volume :
215
Co-Authors:
Gidoni, D., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Brosio, P., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Bond-Nutter, D., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Bedbrook, J., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Dunsmuir, P., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Facilitators :
From page:
337
To page:
344
(
Total pages:
8
)
Abstract:
In order to identify specific cis-acting elements which regulate the expression of the divergent Cab22R and Cab22L genes of Petunia, we conducted systematic mutational studies of the 1 kb intergenic promoter region. Sequence analysis revealed three GATA box sequence repeats positioned between the TATA and CAAT box elements. These GATA elements are conserved in corresponding promoter regions of all LHCII Type I Cab genes in Petunia and other dicotyledonous plants we have examined. Sitespecific mutations in the CAAT box and the GATA box elements of the Cab22R promoter resulted in 8-fold and 5-fold reductions in Cab22R transcript levels respectively. A deletion of 52 bp, adjacent and upstream from the CAAT box (-92 to- 145) in the Cab22R promoter reduced transcript levels 20-fold. This deletion contains a region of 13 pb which is conserved between many Petunia Cab genes. These results indicate that the quantitative expression of the Cab22 promoters is regulated by multiple cis-acting elements including CAAT and GATA box elements as well as sequences located between -92 and -145. The deletion of the region between -92 and -145 is partially compensted by homologous sequences present in the adjacent divergent promoter Cab22L. © 1989 Springer-Verlag.
Note:
Related Files :
chlorophyll
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molecular genetics
mutation
photosynthesis
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More details
DOI :
10.1007/BF00339739
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30705
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
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Scientific Publication
Novel cis-acting elements in petunia Cab gene promoters
215
Gidoni, D., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Brosio, P., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Bond-Nutter, D., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Bedbrook, J., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Dunsmuir, P., Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, Oakland, 94611, CA, United States
Novel cis-acting elements in petunia Cab gene promoters
In order to identify specific cis-acting elements which regulate the expression of the divergent Cab22R and Cab22L genes of Petunia, we conducted systematic mutational studies of the 1 kb intergenic promoter region. Sequence analysis revealed three GATA box sequence repeats positioned between the TATA and CAAT box elements. These GATA elements are conserved in corresponding promoter regions of all LHCII Type I Cab genes in Petunia and other dicotyledonous plants we have examined. Sitespecific mutations in the CAAT box and the GATA box elements of the Cab22R promoter resulted in 8-fold and 5-fold reductions in Cab22R transcript levels respectively. A deletion of 52 bp, adjacent and upstream from the CAAT box (-92 to- 145) in the Cab22R promoter reduced transcript levels 20-fold. This deletion contains a region of 13 pb which is conserved between many Petunia Cab genes. These results indicate that the quantitative expression of the Cab22 promoters is regulated by multiple cis-acting elements including CAAT and GATA box elements as well as sequences located between -92 and -145. The deletion of the region between -92 and -145 is partially compensted by homologous sequences present in the adjacent divergent promoter Cab22L. © 1989 Springer-Verlag.
Scientific Publication
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