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Factors affecting success of embryo collection and transfer in a transgenic coat program
Year:
1997
Source of publication :
Theriogenology
Authors :
Barash, Itamar
;
.
Bor, Amir
;
.
Dekel, Itzhak
;
.
Friedler, A.
;
.
Gootwine, Elisha
;
.
Heller, M.
;
.
Shani, Moshe
;
.
Zaharoni, U.
;
.
Zenou, Abraham
;
.
Volume :
48
Co-Authors:
Gootwine, E., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Barash, I., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Bor, A., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Dekel, I., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Friedler, A., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Heller, M., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Zaharoni, U., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Zenue, A., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Shani, M., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Facilitators :
From page:
485
To page:
499
(
Total pages:
15
)
Abstract:
During a goat transgenic program that took place in Israel from July 1995 to February 1996, Saanen (n=343) and Nubian x Damascus (n=378) crossbred goats of mixed ages were used as donors (n=433) and recipients (n=288). The effects of season, age, number of surgical procedures, previous hormonal treatments and ovulation rate on the number of microinjectable embryos collected were studied. Likewise, the effects of these parameters on the pregnancy rate as well as the number of embryos transplanted, endogenous progesterone concentrations and exogenous progesterone supplementation were studied in recipient does. Following superovulation with ovine follicle stimulating hormone, 85% of the does responded with 13.6±5.7 (mean±S D) ovulations/doe. Age, month and number of previous hormonal treatments significantly affected the ovulation rate. The average recovery rate was 70%, and it was affected only by the ovulation rate. Pronuclei were visualized in about 30% of the flushed embryos (including unfertilized ova), and those were microinjected with human serum albumin gene construct. About 68% of the injected embryos underwent at least one division during an overnight incubation, and those embryos were transferred, giving about 2.0 transferred embryos per ovulated donor. Of the recipients, 86% responded following synchronization with 3.1±1.6 (mean±S D) ovulations per doe. Breed and month had a significant effect on the ovulation rate. Two or three microinjected embryos were transferred to each recipient, resulting in more than a 40% pregnancy rate during September to November. Lower pregnancy rates were obtained before and after that period. By monitoring plasma progesterone concentrations in the recipients it was found that progesterone concentration was correlated with the ovulation rate. However, the pregnancy rate was not affected by progesterone concentration. During January and February, 30 to 50% of the recipients failed to develop functional corpora lutea (CL) following embryo transfer, which explained the lower pregnancy rate in those months. Of the 86 kids born 4 were transgenic.
Note:
Related Files :
animal cell
animal experiment
animal tissue
corpus luteum
Female
goats
ovulation
progesterone blood level
recipient
Superovulation
Show More
Related Content
More details
DOI :
10.1016/S0093-691X(97)00257-4
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30731
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
You may also be interested in
Scientific Publication
Factors affecting success of embryo collection and transfer in a transgenic coat program
48
Gootwine, E., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Barash, I., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Bor, A., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Dekel, I., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Friedler, A., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Heller, M., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Zaharoni, U., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Zenue, A., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Shani, M., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50 250, Israel
Factors affecting success of embryo collection and transfer in a transgenic coat program
During a goat transgenic program that took place in Israel from July 1995 to February 1996, Saanen (n=343) and Nubian x Damascus (n=378) crossbred goats of mixed ages were used as donors (n=433) and recipients (n=288). The effects of season, age, number of surgical procedures, previous hormonal treatments and ovulation rate on the number of microinjectable embryos collected were studied. Likewise, the effects of these parameters on the pregnancy rate as well as the number of embryos transplanted, endogenous progesterone concentrations and exogenous progesterone supplementation were studied in recipient does. Following superovulation with ovine follicle stimulating hormone, 85% of the does responded with 13.6±5.7 (mean±S D) ovulations/doe. Age, month and number of previous hormonal treatments significantly affected the ovulation rate. The average recovery rate was 70%, and it was affected only by the ovulation rate. Pronuclei were visualized in about 30% of the flushed embryos (including unfertilized ova), and those were microinjected with human serum albumin gene construct. About 68% of the injected embryos underwent at least one division during an overnight incubation, and those embryos were transferred, giving about 2.0 transferred embryos per ovulated donor. Of the recipients, 86% responded following synchronization with 3.1±1.6 (mean±S D) ovulations per doe. Breed and month had a significant effect on the ovulation rate. Two or three microinjected embryos were transferred to each recipient, resulting in more than a 40% pregnancy rate during September to November. Lower pregnancy rates were obtained before and after that period. By monitoring plasma progesterone concentrations in the recipients it was found that progesterone concentration was correlated with the ovulation rate. However, the pregnancy rate was not affected by progesterone concentration. During January and February, 30 to 50% of the recipients failed to develop functional corpora lutea (CL) following embryo transfer, which explained the lower pregnancy rate in those months. Of the 86 kids born 4 were transgenic.
Scientific Publication
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