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Shoot production in squash (Cucurbita pepo) by in vitro organogenesis
Year:
2003
Source of publication :
Plant Cell Reports
Authors :
Elman, Chassia
;
.
Gaba, Victor
;
.
Gal-On, Amit
;
.
Paris, Harry
;
.
Singer, Sima
;
.
Xia, Xiaodi
;
.
Volume :
21
Co-Authors:
Ananthakrishnan, G., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel, University of Florida, Citrus Research and Education Center, 700 Experiment Station Rd, Lake Alfred, FL 33850-2299, United States
Xia, X., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Elman, C., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Singer, S., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Paris, H.S., Department of Vegetable Crops, ARO Newe Ya'ar Research Center, P.O. Box 1021, 30095 Ramat Yishay, Israel
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Gaba, V., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
739
To page:
746
(
Total pages:
8
)
Abstract:
Seedling-derived cotyledon explants of squash (Cucurbita pepo L.) of commercial cultivars True French, Ma'yan and Goldy were regenerated in vitro on Murashige and Skoog medium augmented with 1 mg/l benzyladenine. After 4 weeks in culture small shoots and buds regenerated only on the most proximal cotyledon edge. Culture on an elongation medium with a reduced cytokinin concentration (0.1 mg/l) with or without 1 mg/l gibberellic acid (GA3) facilitated the recovery of shoots. Fresh shoots could be recovered at each subculture of the regenerating mass. Peak productivity was during the third cycle of subculture, and shoot production ceased after the fifth subculture. Culture on elongation medium supplemented with GA3 was 55% more effective with respect to overall shoot production than that on medium without GA3, with 22 shoots recovered in total per explant from the former. Regeneration occurred under both light and dark conditions. All of the shoots tested were diploid. The shoots were rooted and transferred to the greenhouse where they grew and flowered normally.
Note:
Related Files :
Cotyledon
Cucurbita
Cucurbita pepo
Direct organogenesis
gibberellins
light
Seed
Shoot elongation
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30839
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:57
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Scientific Publication
Shoot production in squash (Cucurbita pepo) by in vitro organogenesis
21
Ananthakrishnan, G., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel, University of Florida, Citrus Research and Education Center, 700 Experiment Station Rd, Lake Alfred, FL 33850-2299, United States
Xia, X., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Elman, C., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Singer, S., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Paris, H.S., Department of Vegetable Crops, ARO Newe Ya'ar Research Center, P.O. Box 1021, 30095 Ramat Yishay, Israel
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Gaba, V., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Shoot production in squash (Cucurbita pepo) by in vitro organogenesis
Seedling-derived cotyledon explants of squash (Cucurbita pepo L.) of commercial cultivars True French, Ma'yan and Goldy were regenerated in vitro on Murashige and Skoog medium augmented with 1 mg/l benzyladenine. After 4 weeks in culture small shoots and buds regenerated only on the most proximal cotyledon edge. Culture on an elongation medium with a reduced cytokinin concentration (0.1 mg/l) with or without 1 mg/l gibberellic acid (GA3) facilitated the recovery of shoots. Fresh shoots could be recovered at each subculture of the regenerating mass. Peak productivity was during the third cycle of subculture, and shoot production ceased after the fifth subculture. Culture on elongation medium supplemented with GA3 was 55% more effective with respect to overall shoot production than that on medium without GA3, with 22 shoots recovered in total per explant from the former. Regeneration occurred under both light and dark conditions. All of the shoots tested were diploid. The shoots were rooted and transferred to the greenhouse where they grew and flowered normally.
Scientific Publication
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