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Metabolic networking in Brunfelsia calycina petals after flower opening
Year:
2010
Source of publication :
Journal of Experimental Botany
Authors :
Bar, Einat
;
.
Bar-Akiva, Ayelet
;
.
Bar-Or, Carmiya
;
.
Freiman, Zohar E.
;
.
Gollop, Natan
;
.
Koltai, Hinanit
;
.
Lewinsohn, Efraim
;
.
Nissim-Levi, Ada
;
.
Oren-Shamir, Michal
;
.
Ovadia, Rinat
;
.
Volume :
61
Co-Authors:
Bar-Akiva, A., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Ovadia, R., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Rogachev, I., Department of Plant Sciences, Weizmann Organization of Science, Rehovot 76100, Israel
Bar-Or, C., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Bar, E., Department of Vegetable Crops, Agriculture Research Organization, Newe ya'Ar Research Center, PO Box 1021, Ramat Yishay 30095, Israel
Freiman, Z., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Nissim-Levi, A., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Gollop, N., Department of Food Science, Agriculture Research Institute, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lewinsohn, E., Department of Vegetable Crops, Agriculture Research Organization, Newe ya'Ar Research Center, PO Box 1021, Ramat Yishay 30095, Israel
Aharoni, A., Department of Plant Sciences, Weizmann Organization of Science, Rehovot 76100, Israel
Weiss, D., Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Koltai, H., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Oren-Shamir, M., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
1393
To page:
1403
(
Total pages:
11
)
Abstract:
Brunfelsia calycina flowers change colour from purple to white due to anthocyanin degradation, parallel to an increase in fragrance and petal size. Here it was tested whether the production of the fragrant benzenoids is dependent on induction of the shikimate pathway, or if they are formed from the anthocyanin degradation products. An extensive characterization of the events taking place in Brunfelsia flowers is presented. Anthocyanin characterization was performed using ultraperfomance liquid chromatography-quadrupole time of flight-tandem mass specrometry (UPLC-QTOF-MS/MS). Volatiles emitted were identified by headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). Accumulated proteins were identified by 2D gel electrophoresis. Transcription profiles were characterized by cross-species hybridization of Brunfelsia cDNAs to potato cDNA microarrays. Identification of accumulated metabolites was performed by UPLC-QTOF-MS non-targeted metabolite analysis. The results include characterization of the nine main anthocyanins in Brunfelsia flowers. In addition, 146 up-regulated genes, 19 volatiles, seven proteins, and 17 metabolites that increased during anthocyanin degradation were identified. A multilevel analysis suggests induction of the shikimate pathway. This pathway is the most probable source of the phenolic acids, which in turn are precursors of both the benzenoid and lignin production pathways. The knowledge obtained is valuable for future studies on degradation of anthocyanins, formation of volatiles, and the network of secondary metabolism in Brunfelsia and related species.
Note:
Related Files :
angiosperm
Brunfelsia
Calycina
metabolism
Models, Biological
Solanaceae
Solanum tuberosum
Show More
Related Content
More details
DOI :
10.1093/jxb/erq008
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30844
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:57
Scientific Publication
Metabolic networking in Brunfelsia calycina petals after flower opening
61
Bar-Akiva, A., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Ovadia, R., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Rogachev, I., Department of Plant Sciences, Weizmann Organization of Science, Rehovot 76100, Israel
Bar-Or, C., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Bar, E., Department of Vegetable Crops, Agriculture Research Organization, Newe ya'Ar Research Center, PO Box 1021, Ramat Yishay 30095, Israel
Freiman, Z., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Nissim-Levi, A., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Gollop, N., Department of Food Science, Agriculture Research Institute, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lewinsohn, E., Department of Vegetable Crops, Agriculture Research Organization, Newe ya'Ar Research Center, PO Box 1021, Ramat Yishay 30095, Israel
Aharoni, A., Department of Plant Sciences, Weizmann Organization of Science, Rehovot 76100, Israel
Weiss, D., Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Koltai, H., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Oren-Shamir, M., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Metabolic networking in Brunfelsia calycina petals after flower opening
Brunfelsia calycina flowers change colour from purple to white due to anthocyanin degradation, parallel to an increase in fragrance and petal size. Here it was tested whether the production of the fragrant benzenoids is dependent on induction of the shikimate pathway, or if they are formed from the anthocyanin degradation products. An extensive characterization of the events taking place in Brunfelsia flowers is presented. Anthocyanin characterization was performed using ultraperfomance liquid chromatography-quadrupole time of flight-tandem mass specrometry (UPLC-QTOF-MS/MS). Volatiles emitted were identified by headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). Accumulated proteins were identified by 2D gel electrophoresis. Transcription profiles were characterized by cross-species hybridization of Brunfelsia cDNAs to potato cDNA microarrays. Identification of accumulated metabolites was performed by UPLC-QTOF-MS non-targeted metabolite analysis. The results include characterization of the nine main anthocyanins in Brunfelsia flowers. In addition, 146 up-regulated genes, 19 volatiles, seven proteins, and 17 metabolites that increased during anthocyanin degradation were identified. A multilevel analysis suggests induction of the shikimate pathway. This pathway is the most probable source of the phenolic acids, which in turn are precursors of both the benzenoid and lignin production pathways. The knowledge obtained is valuable for future studies on degradation of anthocyanins, formation of volatiles, and the network of secondary metabolism in Brunfelsia and related species.
Scientific Publication
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