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Problems associated with in vitro culture propagation and virus detection
Year:
2006
Source of publication :
Acta Horticulturae
Authors :
Spiegel, Sara
;
.
Volume :
722
Co-Authors:
Facilitators :
From page:
79
To page:
82
(
Total pages:
4
)
Abstract:
In vitro grown plant cultures are being utilized for commercial propagation, conservation of germplasm collections, preservation of plant clones and even virus isolates in host plants. During the establishment of in vitro cultures, pests like fungi and bacteria are eliminated or minimized, whereas viruses are not. Detection of viral agents in in vitro grown plant cultures is achieved mainly by ELISA and assays based on the polymerase chain reaction (PCR) technique. These methods are in general reliable and sensitive, however, in some host-virus systems detection is still a challenge. The goal of this paper is to discuss the importance of pathogen detection in plant cultures grown in vitro with special attention to viruses found in low levels in infected plantlets.
Note:
Related Files :
Bacteria (microorganisms)
fungi
Germplasm collection
micropropagation
PCR
Polymerase Chain Reaction
virus detection
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Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
30865
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:57
Scientific Publication
Problems associated with in vitro culture propagation and virus detection
722
Problems associated with in vitro culture propagation and virus detection
In vitro grown plant cultures are being utilized for commercial propagation, conservation of germplasm collections, preservation of plant clones and even virus isolates in host plants. During the establishment of in vitro cultures, pests like fungi and bacteria are eliminated or minimized, whereas viruses are not. Detection of viral agents in in vitro grown plant cultures is achieved mainly by ELISA and assays based on the polymerase chain reaction (PCR) technique. These methods are in general reliable and sensitive, however, in some host-virus systems detection is still a challenge. The goal of this paper is to discuss the importance of pathogen detection in plant cultures grown in vitro with special attention to viruses found in low levels in infected plantlets.
Scientific Publication
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