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Distribution of Xylella fastidiosa in blueberry stem and root sections in relation to disease severity in the field
Year:
2014
Source of publication :
Plant Disease
Authors :
Gamliel-Atinsky, Efrat
;
.
Volume :
98
Co-Authors:
Holland, R.M., Department of Plant Pathology, University of Georgia, Athens 30602, United States, University of Georgia, Bacon County Cooperative Extension, Alma 31510, United States
Christiano, R.S.C., Department of Plant Pathology, University of Georgia, Athens 30602, United States, University of California-Riverside, Lindcove Research and Extension Center, Exeter 93221, United Kingdom
Gamliel-Atinsky, E., Department of Plant Pathology, University of Georgia, Athens 30602, United States
Scherm, H., Department of Plant Pathology, University of Georgia, Athens 30602, United States
Facilitators :
From page:
443
To page:
447
(
Total pages:
5
)
Abstract:
Xylella fastidiosa causes bacterial leaf scorch, a new disease of southern highbush blueberry in the southeastern United States. Infections occlude the xylem of affected plants, causing drought-like symptoms and, eventually, plant death. To assess the likelihood of mitigation of bacterial leaf scorch through cultural practices such as pruning or hedging of affected plants, we determined the localization and population density of X. fastidiosa in naturally infected blueberry plants with varying levels of bacterial leaf scorch severity. Stem segments were sampled from the current season's growth down to the base of the plant, as were root segments on plants that were either asymptomatic or had light, moderate, or severe symptoms in three plantings affected by the disease. Stem sap was extracted from each segment and population densities of X. fastidiosa were determined using real-time polymerase chain reaction with species-specific primers. Detection frequencies were lowest (but non-zero) in sap from asymptomatic plants and highest in plants with severe symptoms. In asymptomatic plants, detection was generally least frequent (0 to 20.0%) in top and root sections and highest (4.6 to 55.6%) in middle and base stem sections. As disease severity increased, detection frequencies in roots increased to >80% in two plantings and to 60% in the third planting. Overall, detection frequencies were highest (>80%) in middle and base stem sections of plants from the moderate and severe disease classes. The lowest bacterial titers (averaging 0 to 2.1 × 101 CFU per 50 μl of sap) were observed in top and root sections of asymptomatic plants, whereas the highest titers (generally between 104 and 105 CFU per 50 μl of sap) were obtained from middle, base, and root sections of plants from the moderate and severe classes. The presence of the bacterium in middle and base stem sections at low disease severity indicates rapid distribution of X. fastidiosa in affected plants. Because the pathogen accumulates in the roots at moderate and high disease severity levels, management strategies such as pruning and mowing are unlikely to be effective in curing affected plants from bacterial leaf scorch. © 2014 The American Phytopathological Society.
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DOI :
10.1094/PDIS-06-13-0680-RE
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30887
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
Scientific Publication
Distribution of Xylella fastidiosa in blueberry stem and root sections in relation to disease severity in the field
98
Holland, R.M., Department of Plant Pathology, University of Georgia, Athens 30602, United States, University of Georgia, Bacon County Cooperative Extension, Alma 31510, United States
Christiano, R.S.C., Department of Plant Pathology, University of Georgia, Athens 30602, United States, University of California-Riverside, Lindcove Research and Extension Center, Exeter 93221, United Kingdom
Gamliel-Atinsky, E., Department of Plant Pathology, University of Georgia, Athens 30602, United States
Scherm, H., Department of Plant Pathology, University of Georgia, Athens 30602, United States
Distribution of Xylella fastidiosa in blueberry stem and root sections in relation to disease severity in the field
Xylella fastidiosa causes bacterial leaf scorch, a new disease of southern highbush blueberry in the southeastern United States. Infections occlude the xylem of affected plants, causing drought-like symptoms and, eventually, plant death. To assess the likelihood of mitigation of bacterial leaf scorch through cultural practices such as pruning or hedging of affected plants, we determined the localization and population density of X. fastidiosa in naturally infected blueberry plants with varying levels of bacterial leaf scorch severity. Stem segments were sampled from the current season's growth down to the base of the plant, as were root segments on plants that were either asymptomatic or had light, moderate, or severe symptoms in three plantings affected by the disease. Stem sap was extracted from each segment and population densities of X. fastidiosa were determined using real-time polymerase chain reaction with species-specific primers. Detection frequencies were lowest (but non-zero) in sap from asymptomatic plants and highest in plants with severe symptoms. In asymptomatic plants, detection was generally least frequent (0 to 20.0%) in top and root sections and highest (4.6 to 55.6%) in middle and base stem sections. As disease severity increased, detection frequencies in roots increased to >80% in two plantings and to 60% in the third planting. Overall, detection frequencies were highest (>80%) in middle and base stem sections of plants from the moderate and severe disease classes. The lowest bacterial titers (averaging 0 to 2.1 × 101 CFU per 50 μl of sap) were observed in top and root sections of asymptomatic plants, whereas the highest titers (generally between 104 and 105 CFU per 50 μl of sap) were obtained from middle, base, and root sections of plants from the moderate and severe classes. The presence of the bacterium in middle and base stem sections at low disease severity indicates rapid distribution of X. fastidiosa in affected plants. Because the pathogen accumulates in the roots at moderate and high disease severity levels, management strategies such as pruning and mowing are unlikely to be effective in curing affected plants from bacterial leaf scorch. © 2014 The American Phytopathological Society.
Scientific Publication
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