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The site of inhibition of the chloroplast electron‐transport system by 2,3‐dithiopropan‐1‐ol (BAL)
Year:
1987
Source of publication :
European Journal of Biochemistry
Authors :
Shahak, Yosepha
;
.
Volume :
164
Co-Authors:
SHAHAK, Y., Biochemistry Department, Weizmann Institute of Science, Rehovot, Israel
HIND, G., Biology Department, Brookhaven National Laboratory, Upton, New York, United States
PADAN, E., Divison of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University, Jerusalem, Israel
Facilitators :
From page:
453
To page:
460
(
Total pages:
8
)
Abstract:
BAL (2,3‐dithiopropan‐1‐ol) treatment of chloroplasts has previously been reported to induce a block in electron transport from water to NADP+ at a site preceding plastocyanin [Belkin et al. (1980) Biochim. Biophys. Acta 766, 563–569]. In the present work the block was further characterized. The following properties of BAL treatment are described. a) Inhibition of electron transport from water to lipophilic acceptors but not to silicomolybdate. b) Inhibition of the slow, sigmoidal phase of chlorophyll a fluorescence induction. c) Inability of N, N, N′,N′,‐tetramethyl‐p‐phenylenediamine to bypass the inhibition of NADP+ photoreduction with water as the electron donor. d) Inhibition of electron transport from externally added quinols to NADP+ e) Inhibition of cytochrome f reduction by photosystem II, but not its oxidation by photosystem I. f) Inhibition of cytochrome b6 turnover and cytochrome f rereduction after single‐turnover flash illumination under cyclic electron‐flow conditions. The BAL‐induced block is therefore located between the secondary quinone acceptor (QB) and the cytochrome b6f complex. It was further found that (a) the isolated cytochrome complex is not inhibited after BAL treatment; (b) BAL‐reacted plastoquinone‐1 inhibits electron transport in chloroplasts; (c) BAL does not inhibit electron transport in chromatophores of Rhodospirilum rubrum or Rhodopseudomonas capsulata. It is suggested that the inhibition of electron transport in chloroplasts results from specific reaction of BAL with the endogenous plastoquinone. Copyright © 1987, Wiley Blackwell. All rights reserved
Note:
Related Files :
Chloroplasts
cytochrome
Cytochromes
drug effect
metabolism
plant
Plants
Rhodopseudomonas
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More details
DOI :
10.1111/j.1432-1033.1987.tb11078.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30903
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
Scientific Publication
The site of inhibition of the chloroplast electron‐transport system by 2,3‐dithiopropan‐1‐ol (BAL)
164
SHAHAK, Y., Biochemistry Department, Weizmann Institute of Science, Rehovot, Israel
HIND, G., Biology Department, Brookhaven National Laboratory, Upton, New York, United States
PADAN, E., Divison of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University, Jerusalem, Israel
The site of inhibition of the chloroplast electron‐transport system by 2,3‐dithiopropan‐1‐ol (BAL)
BAL (2,3‐dithiopropan‐1‐ol) treatment of chloroplasts has previously been reported to induce a block in electron transport from water to NADP+ at a site preceding plastocyanin [Belkin et al. (1980) Biochim. Biophys. Acta 766, 563–569]. In the present work the block was further characterized. The following properties of BAL treatment are described. a) Inhibition of electron transport from water to lipophilic acceptors but not to silicomolybdate. b) Inhibition of the slow, sigmoidal phase of chlorophyll a fluorescence induction. c) Inability of N, N, N′,N′,‐tetramethyl‐p‐phenylenediamine to bypass the inhibition of NADP+ photoreduction with water as the electron donor. d) Inhibition of electron transport from externally added quinols to NADP+ e) Inhibition of cytochrome f reduction by photosystem II, but not its oxidation by photosystem I. f) Inhibition of cytochrome b6 turnover and cytochrome f rereduction after single‐turnover flash illumination under cyclic electron‐flow conditions. The BAL‐induced block is therefore located between the secondary quinone acceptor (QB) and the cytochrome b6f complex. It was further found that (a) the isolated cytochrome complex is not inhibited after BAL treatment; (b) BAL‐reacted plastoquinone‐1 inhibits electron transport in chloroplasts; (c) BAL does not inhibit electron transport in chromatophores of Rhodospirilum rubrum or Rhodopseudomonas capsulata. It is suggested that the inhibition of electron transport in chloroplasts results from specific reaction of BAL with the endogenous plastoquinone. Copyright © 1987, Wiley Blackwell. All rights reserved
Scientific Publication
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