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Serological detection of grapevine virus A using antiserum to a nonstructural protein, the putative movement protein
Year:
1997
Source of publication :
Phytopathology
Authors :
Gafny, Ron
;
.
Rubinson, Elena
;
.
Volume :
87
Co-Authors:
Rubinson, E., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Galiakparov, N., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Radian, S., Virus Laboratory, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sela, I., Virus Laboratory, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Tanne, E., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Gafny, R., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
1041
To page:
1045
(
Total pages:
5
)
Abstract:
Grapevine virus A (GVA) is implicated in the etiology of the rugose wood disease. The coat protein (CP) and the putative movement protein (MP) genes of GVA were cloned and expressed in Escherichia coli and used to produce antisera. Both the CP and the MP were detected with their corresponding antisera in GVA-infected Nicotiana benthamiana. The MP was first detected at an early stage of the infection, 6 to 12 h after inoculation, and the CP was detected 2 to 3 days after inoculation. The CP and MP were detected by immunoblot analysis in rugose wood-affected grapevines. The MP could be detected in GVA-infected grapevines that tested negative for CP, both with CP antiserum and with a commercially available enzyme-linked immunosorbent assay kit. The study shows that detection of the nonstructural MP may be an effective means for serological detection of GVA infection in grapevines.
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DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30944
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
Scientific Publication
Serological detection of grapevine virus A using antiserum to a nonstructural protein, the putative movement protein
87
Rubinson, E., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Galiakparov, N., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Radian, S., Virus Laboratory, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sela, I., Virus Laboratory, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Tanne, E., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Gafny, R., Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Serological detection of grapevine virus A using antiserum to a nonstructural protein, the putative movement protein
Grapevine virus A (GVA) is implicated in the etiology of the rugose wood disease. The coat protein (CP) and the putative movement protein (MP) genes of GVA were cloned and expressed in Escherichia coli and used to produce antisera. Both the CP and the MP were detected with their corresponding antisera in GVA-infected Nicotiana benthamiana. The MP was first detected at an early stage of the infection, 6 to 12 h after inoculation, and the CP was detected 2 to 3 days after inoculation. The CP and MP were detected by immunoblot analysis in rugose wood-affected grapevines. The MP could be detected in GVA-infected grapevines that tested negative for CP, both with CP antiserum and with a commercially available enzyme-linked immunosorbent assay kit. The study shows that detection of the nonstructural MP may be an effective means for serological detection of GVA infection in grapevines.
Scientific Publication
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