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Plant respiratory burst oxidase homologs impinge on wound responsiveness and development in Lycopersicon esculentum
Year:
2004
Source of publication :
Plant Cell
Authors :
Ophir, Ron
;
.
Volume :
16
Co-Authors:
Sagi, M., Institute for Applied Research, Ben-Gurion University, Beer Sheva 84105, Israel
Davydov, O., Department of Plant Science, Weizmann Institute of Science, Rehovot 76100, Israel
Orazova, S., Institute for Applied Research, Ben-Gurion University, Beer Sheva 84105, Israel
Yesbergenova, Z., Institute for Applied Research, Ben-Gurion University, Beer Sheva 84105, Israel
Ophir, R., Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel
Stratmann, J.W., Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, United States
Fluhr, R., Department of Plant Science, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
616
To page:
628
(
Total pages:
13
)
Abstract:
Plant respiratory burst oxidase homologs (Rboh) are homologs of the human neutrophil pathogen-related gp91phox. Antisense technology was employed to ascertain the biological function of Lycopersicon esculentum (tomato) Rboh. Lines with diminished Rboh activity showed a reduced level of reactive oxygen species (ROS) in the leaf, implying a role for Rboh in establishing the cellular redox milieu. Surprisingly, the antisense plants acquired a highly branched phenotype, switched from indeterminate to determinate growth habit, and had fasciated reproductive organs. Wound-induced systemic expression of proteinase inhibitor II was compromised in the antisense lines, indicating that ROS intermediates supplied by Rboh are required for this wound response. Extending these observations by transcriptome analysis revealed ectopic leaf expression of homeotic MADS box genes that are normally expressed only in reproductive organs. In addition, both Rboh-dependent and -independent wound-induced gene induction was detected as well as transcript changes related to redox maintenance. The results provide novel insights into how the steady state cellular level of ROS is controlled and portrays the role of Rboh as a signal transducer of stress and developmental responses.
Note:
Related Files :
enzymes
Gene
Genes
Genetics
metabolism
phenotype
reactive oxygen species
redox reactions
Show More
Related Content
More details
DOI :
10.1105/tpc.019398
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30955
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
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Scientific Publication
Plant respiratory burst oxidase homologs impinge on wound responsiveness and development in Lycopersicon esculentum
16
Sagi, M., Institute for Applied Research, Ben-Gurion University, Beer Sheva 84105, Israel
Davydov, O., Department of Plant Science, Weizmann Institute of Science, Rehovot 76100, Israel
Orazova, S., Institute for Applied Research, Ben-Gurion University, Beer Sheva 84105, Israel
Yesbergenova, Z., Institute for Applied Research, Ben-Gurion University, Beer Sheva 84105, Israel
Ophir, R., Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel
Stratmann, J.W., Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, United States
Fluhr, R., Department of Plant Science, Weizmann Institute of Science, Rehovot 76100, Israel
Plant respiratory burst oxidase homologs impinge on wound responsiveness and development in Lycopersicon esculentum
Plant respiratory burst oxidase homologs (Rboh) are homologs of the human neutrophil pathogen-related gp91phox. Antisense technology was employed to ascertain the biological function of Lycopersicon esculentum (tomato) Rboh. Lines with diminished Rboh activity showed a reduced level of reactive oxygen species (ROS) in the leaf, implying a role for Rboh in establishing the cellular redox milieu. Surprisingly, the antisense plants acquired a highly branched phenotype, switched from indeterminate to determinate growth habit, and had fasciated reproductive organs. Wound-induced systemic expression of proteinase inhibitor II was compromised in the antisense lines, indicating that ROS intermediates supplied by Rboh are required for this wound response. Extending these observations by transcriptome analysis revealed ectopic leaf expression of homeotic MADS box genes that are normally expressed only in reproductive organs. In addition, both Rboh-dependent and -independent wound-induced gene induction was detected as well as transcript changes related to redox maintenance. The results provide novel insights into how the steady state cellular level of ROS is controlled and portrays the role of Rboh as a signal transducer of stress and developmental responses.
Scientific Publication
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