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An improved large-scale screening method for assessment of colletotrichum coccodes aggressiveness using mature green tomatoes
Year:
2009
Source of publication :
Plant Pathology
Authors :
Tsror, Leah
;
.
Volume :
58
Co-Authors:
Ben-Daniel, B., Department of Plant Pathology, Agricultural Research Organization, Gilat Research Center, M. P. Negev 85280, Israel, Department of Life Sciences, Ben-Gurion University, Be'er-Sheva, Israel
Bar-Zvi, D., Department of Life Sciences, Ben-Gurion University, Be'er-Sheva, Israel
Tsror, L., Department of Plant Pathology, Agricultural Research Organization, Gilat Research Center, M. P. Negev 85280, Israel
Facilitators :
From page:
497
To page:
503
(
Total pages:
7
)
Abstract:
A reliable, rapid and low-cost screening bioassay, based on measuring lesion sizes, rotten tissue weight and tissue colonization 1 week after the injection of a conidial suspension into the stem scars of mature green tomato fruits, was developed to assess the aggressiveness of Colletotrichum coccodes isolates. This protocol was compared with inoculation of either potato plantlets from in vitro multiplication, or ripe tomato fruits, using C. coccodes isolates from potato and from tomato. Aggressiveness to mature green tomato fruits was scored by measuring lesion size and weight at the scar end of the fruit, while colonization was measured in samples taken from the blossom end. Values of all three disease parameters were significantly higher (ca. 3 times) with aggressive isolates of the pathogen than with less aggressive ones. High correlation levels among these three parameters were obtained. Also, the aggressiveness to mature green tomatoes was highly correlated with the aggressiveness to potato tissue culture plantlets. It was concluded that lesion size in inoculated tomatoes can be used as a sole measure for estimating the aggressiveness of C. coccodes isolates to either tomato or potato. © 2008 BSPP.
Note:
Related Files :
Anthracnose
Bioassay
Black dot
Colletotrichum
isolated population
Pathology
Solanum
Solanum lycopersicum
Solanum tuberosum
Show More
Related Content
More details
DOI :
10.1111/j.1365-3059.2008.01989.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30969
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
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Scientific Publication
An improved large-scale screening method for assessment of colletotrichum coccodes aggressiveness using mature green tomatoes
58
Ben-Daniel, B., Department of Plant Pathology, Agricultural Research Organization, Gilat Research Center, M. P. Negev 85280, Israel, Department of Life Sciences, Ben-Gurion University, Be'er-Sheva, Israel
Bar-Zvi, D., Department of Life Sciences, Ben-Gurion University, Be'er-Sheva, Israel
Tsror, L., Department of Plant Pathology, Agricultural Research Organization, Gilat Research Center, M. P. Negev 85280, Israel
An improved large-scale screening method for assessment of colletotrichum coccodes aggressiveness using mature green tomatoes
A reliable, rapid and low-cost screening bioassay, based on measuring lesion sizes, rotten tissue weight and tissue colonization 1 week after the injection of a conidial suspension into the stem scars of mature green tomato fruits, was developed to assess the aggressiveness of Colletotrichum coccodes isolates. This protocol was compared with inoculation of either potato plantlets from in vitro multiplication, or ripe tomato fruits, using C. coccodes isolates from potato and from tomato. Aggressiveness to mature green tomato fruits was scored by measuring lesion size and weight at the scar end of the fruit, while colonization was measured in samples taken from the blossom end. Values of all three disease parameters were significantly higher (ca. 3 times) with aggressive isolates of the pathogen than with less aggressive ones. High correlation levels among these three parameters were obtained. Also, the aggressiveness to mature green tomatoes was highly correlated with the aggressiveness to potato tissue culture plantlets. It was concluded that lesion size in inoculated tomatoes can be used as a sole measure for estimating the aggressiveness of C. coccodes isolates to either tomato or potato. © 2008 BSPP.
Scientific Publication
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