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Interactions between Escherichia coli and the plant-parasitic nematode Meloidogyne javanica
Year:
2008
Source of publication :
Journal of Applied Microbiology
Authors :
Maghodia, Ajay
;
.
Sela, Shlomo
;
.
Spiegel, Yitzhak
;
.
Volume :
105
Co-Authors:
Maghodia, A.B., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Spiegel, Y., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Sela, S., Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, Bet Dagan, Israel, Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
1810
To page:
1816
(
Total pages:
7
)
Abstract:
Aims: To determine the potential of the plant-parasitic nematode Meloidogyne javanica to serve as a temporary reservoir for Escherichia coli. Methods and Results: The adhesion to and persistence of E. coli on the surface of M. javanica were evaluated at different times and temperatures. A pure culture of green fluorescent protein (GFP) tagged E. coli was mixed with ca. 1000 J2 M. javanica for 2 h at 25°C. The nematodes were then washed and the rate of the adhesion of the bacteria to the nematodes was determined by counting the viable nematode-associated E. coli, and by fluorescence microscopy. A dose-dependent adhesion rate was observed only at a bacterium to nematode ratio of 104-106 : 1. The adhesion of E. coli to the nematodes was also tested over a 24 h-period at 4°C, 25°C and 37°C. At 4°C and 37°C, maximal adhesion was observed at 5 h; whereas at 25°C, maximal adherence was observed at 8 h. Survival experiments showed that the bacteria could be detected on the nematodes for up to 2 weeks when incubated at 4°C and 25°C, but not at 37°C. Conclusions: Under laboratory conditions, at 4°C and 25°C, M. javanica could serve as a temporary vector for E. coli for up to 2 weeks. Significance and Impact of the Study: These findings support the hypothesis that, in the presence of high concentrations of E. coli, M. javanica might serve as a potential vehicle for the transmission of food-borne pathogens. © 2008 The Authors.
Note:
Related Files :
adhesion
Animals
bacterium culture
dose-response relationship
Nematoda
Persistence
Plant-parasitic nematodes
temperature
Show More
Related Content
More details
DOI :
10.1111/j.1365-2672.2008.03945.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30974
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
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Scientific Publication
Interactions between Escherichia coli and the plant-parasitic nematode Meloidogyne javanica
105
Maghodia, A.B., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Spiegel, Y., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Sela, S., Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, Bet Dagan, Israel, Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Interactions between Escherichia coli and the plant-parasitic nematode Meloidogyne javanica
Aims: To determine the potential of the plant-parasitic nematode Meloidogyne javanica to serve as a temporary reservoir for Escherichia coli. Methods and Results: The adhesion to and persistence of E. coli on the surface of M. javanica were evaluated at different times and temperatures. A pure culture of green fluorescent protein (GFP) tagged E. coli was mixed with ca. 1000 J2 M. javanica for 2 h at 25°C. The nematodes were then washed and the rate of the adhesion of the bacteria to the nematodes was determined by counting the viable nematode-associated E. coli, and by fluorescence microscopy. A dose-dependent adhesion rate was observed only at a bacterium to nematode ratio of 104-106 : 1. The adhesion of E. coli to the nematodes was also tested over a 24 h-period at 4°C, 25°C and 37°C. At 4°C and 37°C, maximal adhesion was observed at 5 h; whereas at 25°C, maximal adherence was observed at 8 h. Survival experiments showed that the bacteria could be detected on the nematodes for up to 2 weeks when incubated at 4°C and 25°C, but not at 37°C. Conclusions: Under laboratory conditions, at 4°C and 25°C, M. javanica could serve as a temporary vector for E. coli for up to 2 weeks. Significance and Impact of the Study: These findings support the hypothesis that, in the presence of high concentrations of E. coli, M. javanica might serve as a potential vehicle for the transmission of food-borne pathogens. © 2008 The Authors.
Scientific Publication
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