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Bioactive volatiles from an endophytic Daldinia cf. concentrica isolate affect the viability of the plant parasitic nematode Meloidogyne javanica
Year:
2016
Source of publication :
PLoS ONE
Authors :
Bucki, Patricia
;
.
Ezra, David
;
.
Horowitz, Sigal Brown
;
.
Liarzi, Orna
;
.
Volume :
11
Co-Authors:
Liarzi, O., Department of Plant Pathology and Weed Research, ARO - Volcani Center, Rishon LeZion, Israel
Bucki, P., Department of Entomology and the Nematology and Chemistry Units, ARO - Volcani Center, Rishon , LeZion, Israel
Miyara, S.B., Department of Entomology and the Nematology and Chemistry Units, ARO - Volcani Center, Rishon , LeZion, Israel
Ezra, D., Department of Plant Pathology and Weed Research, ARO - Volcani Center, Rishon LeZion, Israel
Facilitators :
From page:
0
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0
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Total pages:
1
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Abstract:
Plant-parasitic nematodes form one of the largest sources of biotic stress imposed on plants, and are very difficult to control; among them are the obligate parasites, the sedentary root-knot nematodes (RKNs)-Meloidogyne spp.-which are extremely polyphagous and exploit a very wide range of hosts. Endophytic fungi are organisms that spend most of their life cycle within plant tissue without causing visible damage to the host plant. Many endophytes secrete specialized metabolites and/or emit volatile organic compounds (VOCs) that exhibit biological activity. Recently, we demonstrated that the endophytic fungus Daldinia cf. concentrica secrets biologically active VOCs. Here we examined the ability of the fungus and its VOCs to control the RKN M. javanica both in vitro and greenhouse experiments. The D. cf. concentrica VOCs showed bionematicidal activity against the second-stage juveniles (J2s) of M. javanica. We found that exposure of J2s to fungal volatiles caused 67% reduction in viability, and that application of a synthetic volatile mixture (SVM), comprising 3-methyl-1- butanol, (-)-2-methyl-1-butanol, 4-heptanone, and isoamyl acetate, in volumetric ratio of 1:1:2:1 further reduced J2s viability by 99%. We demonstrated that, although each of the four VOCs significantly reduced the viability of J2s relative to the control, only 4-heptanone elicited the same effect as the whole mixture, with nematicidal activity of 90% reduction in viability of the J2s. Study of the effect of the SVM on egg hatching demonstrated that it decreased eggs hatching by 87%. Finally, application of the SVM to soil inoculated with M. javanica eggs or J2s prior to planting susceptible tomato plants resulted in a significantly reduced galling index and fewer eggs produced on each root system, with no effect on root weight. Thus, D. cf. concentrica and/or SVM based on fungal VOCs may be considered as a novel alternative approach to controlling the RKN M. javanica. © 2016 Liarzi et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Note:
Related Files :
Animal
Animals
Growth, Development and Aging
Nematoda
Parasitology
Plant Disease
Volatile organic compounds
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More details
DOI :
10.1371/journal.pone.0168437
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30981
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
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Scientific Publication
Bioactive volatiles from an endophytic Daldinia cf. concentrica isolate affect the viability of the plant parasitic nematode Meloidogyne javanica
11
Liarzi, O., Department of Plant Pathology and Weed Research, ARO - Volcani Center, Rishon LeZion, Israel
Bucki, P., Department of Entomology and the Nematology and Chemistry Units, ARO - Volcani Center, Rishon , LeZion, Israel
Miyara, S.B., Department of Entomology and the Nematology and Chemistry Units, ARO - Volcani Center, Rishon , LeZion, Israel
Ezra, D., Department of Plant Pathology and Weed Research, ARO - Volcani Center, Rishon LeZion, Israel
Bioactive volatiles from an endophytic Daldinia cf. concentrica isolate affect the viability of the plant parasitic nematode Meloidogyne javanica
Plant-parasitic nematodes form one of the largest sources of biotic stress imposed on plants, and are very difficult to control; among them are the obligate parasites, the sedentary root-knot nematodes (RKNs)-Meloidogyne spp.-which are extremely polyphagous and exploit a very wide range of hosts. Endophytic fungi are organisms that spend most of their life cycle within plant tissue without causing visible damage to the host plant. Many endophytes secrete specialized metabolites and/or emit volatile organic compounds (VOCs) that exhibit biological activity. Recently, we demonstrated that the endophytic fungus Daldinia cf. concentrica secrets biologically active VOCs. Here we examined the ability of the fungus and its VOCs to control the RKN M. javanica both in vitro and greenhouse experiments. The D. cf. concentrica VOCs showed bionematicidal activity against the second-stage juveniles (J2s) of M. javanica. We found that exposure of J2s to fungal volatiles caused 67% reduction in viability, and that application of a synthetic volatile mixture (SVM), comprising 3-methyl-1- butanol, (-)-2-methyl-1-butanol, 4-heptanone, and isoamyl acetate, in volumetric ratio of 1:1:2:1 further reduced J2s viability by 99%. We demonstrated that, although each of the four VOCs significantly reduced the viability of J2s relative to the control, only 4-heptanone elicited the same effect as the whole mixture, with nematicidal activity of 90% reduction in viability of the J2s. Study of the effect of the SVM on egg hatching demonstrated that it decreased eggs hatching by 87%. Finally, application of the SVM to soil inoculated with M. javanica eggs or J2s prior to planting susceptible tomato plants resulted in a significantly reduced galling index and fewer eggs produced on each root system, with no effect on root weight. Thus, D. cf. concentrica and/or SVM based on fungal VOCs may be considered as a novel alternative approach to controlling the RKN M. javanica. © 2016 Liarzi et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Scientific Publication
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