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Characterization of a 'Bacteroidetes' symbiont in Encarsia wasps (Hymenoptera: Aphelinidae): Proposal of 'Candidatus Cardinium hertigii'
Year:
2004
Authors :
Katzir, Nurit
;
.
Zchori-Fein, Einat
;
.
Volume :
54
Co-Authors:
Zchori-Fein, E., Department of Vegetable Crops, Agricultural Research Oganization, Newe Ya'ar Research Center, Ramat, Yishay 30095, Israel
Perlman, S.J., Department of Entomology, University of Tucson, 410 Forbes Building, Tucson, AZ 85721, United States
Kelly, S.E., Department of Entomology, University of Tucson, 410 Forbes Building, Tucson, AZ 85721, United States
Katzir, N., Department of Vegetable Crops, Agricultural Research Oganization, Newe Ya'ar Research Center, Ramat, Yishay 30095, Israel
Hunter, M.S., Department of Entomology, University of Tucson, 410 Forbes Building, Tucson, AZ 85721, United States
Facilitators :
From page:
961
To page:
968
(
Total pages:
8
)
Abstract:
Previously, analysis of 16S rDNA sequences placed a newly discovered lineage of bacterial symbionts of arthropods in the 'Bacteroidetes'. This symbiont lineage is associated with a number of diverse host reproductive manipulations, including induction of parthenogenesis in several Encarsia parasitoid wasps (Hymenoptera: Aphelinidae). In this study, electron microscopy and phylogenetic analysis of the 16S rRNA and gyrB genes of symbionts from Encarsia hispida and Encarsia pergandiella are used to describe and further characterize these bacteria. Phylogenetic analyses based on these two genes showed that the Encarsia symbionts are allied with the Cytophaga aurantiaca lineage within the 'Bacteroidetes', with their closest described relative being the acanthamoeba symbiont 'Candidatus Amoebophilus asiaticus'. The Encarsia symbionts share 97% 16S rDNA sequence similarity with Brevipalpus mite and Ixodes tick symbionts and 88% sequence similarity with 'Candidatus A. asiaticus'. Electron microscopy revealed that many of the bacteria found in the ovaries of the two Encarsia species contained a regular, brush-like array of microfilament-like structures that appear to be characteristic of the symbiont. Finally, the role of this bacterium in parthenogenesis induction in E. hispida was confirmed. Based on phylogenetic analyses and electron microscopy, classification of the symbionts from Encarsia as 'Candidatus Cardinium hertigii' is proposed. © 2004 IUMS.
Note:
Related Files :
Acari
Amoebophilus asiaticus
Animals
Female
Ixodes
Male
phenotype
Symbiosis
Show More
Related Content
More details
DOI :
10.1099/ijs.0.02957-0
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31046
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:59
You may also be interested in
Scientific Publication
Characterization of a 'Bacteroidetes' symbiont in Encarsia wasps (Hymenoptera: Aphelinidae): Proposal of 'Candidatus Cardinium hertigii'
54
Zchori-Fein, E., Department of Vegetable Crops, Agricultural Research Oganization, Newe Ya'ar Research Center, Ramat, Yishay 30095, Israel
Perlman, S.J., Department of Entomology, University of Tucson, 410 Forbes Building, Tucson, AZ 85721, United States
Kelly, S.E., Department of Entomology, University of Tucson, 410 Forbes Building, Tucson, AZ 85721, United States
Katzir, N., Department of Vegetable Crops, Agricultural Research Oganization, Newe Ya'ar Research Center, Ramat, Yishay 30095, Israel
Hunter, M.S., Department of Entomology, University of Tucson, 410 Forbes Building, Tucson, AZ 85721, United States
Characterization of a 'Bacteroidetes' symbiont in Encarsia wasps (Hymenoptera: Aphelinidae): Proposal of 'Candidatus Cardinium hertigii'
Previously, analysis of 16S rDNA sequences placed a newly discovered lineage of bacterial symbionts of arthropods in the 'Bacteroidetes'. This symbiont lineage is associated with a number of diverse host reproductive manipulations, including induction of parthenogenesis in several Encarsia parasitoid wasps (Hymenoptera: Aphelinidae). In this study, electron microscopy and phylogenetic analysis of the 16S rRNA and gyrB genes of symbionts from Encarsia hispida and Encarsia pergandiella are used to describe and further characterize these bacteria. Phylogenetic analyses based on these two genes showed that the Encarsia symbionts are allied with the Cytophaga aurantiaca lineage within the 'Bacteroidetes', with their closest described relative being the acanthamoeba symbiont 'Candidatus Amoebophilus asiaticus'. The Encarsia symbionts share 97% 16S rDNA sequence similarity with Brevipalpus mite and Ixodes tick symbionts and 88% sequence similarity with 'Candidatus A. asiaticus'. Electron microscopy revealed that many of the bacteria found in the ovaries of the two Encarsia species contained a regular, brush-like array of microfilament-like structures that appear to be characteristic of the symbiont. Finally, the role of this bacterium in parthenogenesis induction in E. hispida was confirmed. Based on phylogenetic analyses and electron microscopy, classification of the symbionts from Encarsia as 'Candidatus Cardinium hertigii' is proposed. © 2004 IUMS.
Scientific Publication
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