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Bacterial community composition and structure of biofilms developing on nanofiltration membranes applied to wastewater treatment
Year:
2007
Source of publication :
Water Research
Authors :
Minz, Dror
;
.
Volvovic, Galit
;
.
Volume :
41
Co-Authors:
Ivnitsky, H., Faculty of Civil and Environmental Engineering, Grand Water Research Institute, Technion, Israel
Katz, I., Faculty of Civil and Environmental Engineering, Grand Water Research Institute, Technion, Israel
Minz, D., Institute of Soil, Water and Environmental Sciences, ARO, The Volcani Center, Bet-Dagan, Israel
Volvovic, G., Institute of Soil, Water and Environmental Sciences, ARO, The Volcani Center, Bet-Dagan, Israel
Shimoni, E., Faculty of Biotechnology and Food Engineering, Technion, IIT Haifa, Israel
Kesselman, E., Faculty of Biotechnology and Food Engineering, Technion, IIT Haifa, Israel
Semiat, R., Faculty of Chemical Engineering, Grand Water Research Institute, Technion, Israel
Dosoretz, C.G., Faculty of Civil and Environmental Engineering, Grand Water Research Institute, Technion, Israel
Facilitators :
From page:
3924
To page:
3935
(
Total pages:
12
)
Abstract:
The structure and microbial communities of biofilms developing on cross-flow nanofiltration (NF) membranes at different temperatures (20, 25 or 34 °C) and operation lengths (8 h-24 days) were studied. Feedwater comprised tertiary quality wastewater effluent or synthetic media mimicking effluents of intermediate quality. After each run, the membranes were autopsied for bacterial enumeration, bacterial community composition and microscopy visualization (SEM, CLSM and AFM/NSOM). Community composition was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) coupled with sequence analysis of 16S rRNA gene fragments from dominant bands. Deposition of polysaccharides and initial bacterial colonization were observed within 8 h, whereas developed biofilms markedly affecting membrane permeability were evident from days 2-3 onwards. Regardless of applied conditions, the heterotrophic plate counts in the biofilm were 3-4×106 CFU/cm2 and the thickness of the biofouling layer was 20-30 μm. From a total of 22 sequences obtained from 14 independent experiments, most species identified were Gram negative (19 of 22 sequences). Proteobacteria were found to be a prevalent group in all cases (16 of 22 sequences) and among it, the β-subclass was the most predominant (8 sequences), followed by the γ-subclass (5 sequences). Pseudomonas/Burkholderia, Ralstonia, Bacteroidetes and Sphingomonas were the dominant groups found in most cases. Even though the microbial population might be important with respect to biofouling patterns, membrane permeability decline seems to be more substantially influenced by the formation and accumulation of exopolymeric substances (EPS). © 2007 Elsevier Ltd. All rights reserved.
Note:
Related Files :
bacteria
electrokinesis
Nanofiltration membranes
nanotechnology
wastewater treatment
water purification
Show More
Related Content
More details
DOI :
10.1016/j.watres.2007.05.021
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31060
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:59
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Scientific Publication
Bacterial community composition and structure of biofilms developing on nanofiltration membranes applied to wastewater treatment
41
Ivnitsky, H., Faculty of Civil and Environmental Engineering, Grand Water Research Institute, Technion, Israel
Katz, I., Faculty of Civil and Environmental Engineering, Grand Water Research Institute, Technion, Israel
Minz, D., Institute of Soil, Water and Environmental Sciences, ARO, The Volcani Center, Bet-Dagan, Israel
Volvovic, G., Institute of Soil, Water and Environmental Sciences, ARO, The Volcani Center, Bet-Dagan, Israel
Shimoni, E., Faculty of Biotechnology and Food Engineering, Technion, IIT Haifa, Israel
Kesselman, E., Faculty of Biotechnology and Food Engineering, Technion, IIT Haifa, Israel
Semiat, R., Faculty of Chemical Engineering, Grand Water Research Institute, Technion, Israel
Dosoretz, C.G., Faculty of Civil and Environmental Engineering, Grand Water Research Institute, Technion, Israel
Bacterial community composition and structure of biofilms developing on nanofiltration membranes applied to wastewater treatment
The structure and microbial communities of biofilms developing on cross-flow nanofiltration (NF) membranes at different temperatures (20, 25 or 34 °C) and operation lengths (8 h-24 days) were studied. Feedwater comprised tertiary quality wastewater effluent or synthetic media mimicking effluents of intermediate quality. After each run, the membranes were autopsied for bacterial enumeration, bacterial community composition and microscopy visualization (SEM, CLSM and AFM/NSOM). Community composition was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) coupled with sequence analysis of 16S rRNA gene fragments from dominant bands. Deposition of polysaccharides and initial bacterial colonization were observed within 8 h, whereas developed biofilms markedly affecting membrane permeability were evident from days 2-3 onwards. Regardless of applied conditions, the heterotrophic plate counts in the biofilm were 3-4×106 CFU/cm2 and the thickness of the biofouling layer was 20-30 μm. From a total of 22 sequences obtained from 14 independent experiments, most species identified were Gram negative (19 of 22 sequences). Proteobacteria were found to be a prevalent group in all cases (16 of 22 sequences) and among it, the β-subclass was the most predominant (8 sequences), followed by the γ-subclass (5 sequences). Pseudomonas/Burkholderia, Ralstonia, Bacteroidetes and Sphingomonas were the dominant groups found in most cases. Even though the microbial population might be important with respect to biofouling patterns, membrane permeability decline seems to be more substantially influenced by the formation and accumulation of exopolymeric substances (EPS). © 2007 Elsevier Ltd. All rights reserved.
Scientific Publication
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