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Cloning and characterization of the Mycobacterium leprae putative ribosomal RNA promoter in Escherichia coli
Year:
1991
Source of publication :
Gene
Authors :
Sela, Shlomo
;
.
Volume :
98
Co-Authors:
Sela, S., Departments of Biology Washington University, St. Louis, MO 63130, United States
Clark-Curtiss, J.E., Departments of Biology Washington University, St. Louis, MO 63130, United States, Departments of Molecular Microbiology, Washington University, St. Louis, MO 63130, United States
Facilitators :
From page:
123
To page:
127
(
Total pages:
5
)
Abstract:
The putative promoter region of the 16S ribosomal RNA-encoding gene (rRNA) of Mycobacterium leprae was cloned and characterized in Escherichia coli. A 932-bp HaeIII restriction fragment, containing the 5' end of the 16S rRNA gene and flanking upstream region, was cloned in front of a promoterless reporter gene in the shuttle vector, pMH109, to generate the plasmid, pYA1101. This clone exhibits promoter activity both in Gram- (E. coli) and Gram+ (Bacillus subtilis) bacteria. Sequence analysis and primer extension experiments with mRNA derived from the M. leprae clone were used to determine the structure and the location of the promoter, as well as the transcription start point in E. coli. The promoter region contains sequences that resemble the -35 and -10 consensus sequences found in many bacteria. A region located 34 bp distal to the promoter is a putative rRNA processing signal, based on sequence homology with processing signals involved in the maturation of the rRNA precursor in B. subtilis and several Mycoplasma species. © 1991.
Note:
Related Files :
16S ribosomal RNA
bacilli
Base Sequence
leprosy
Molecular Sequence Data
Sequence Homology, Nucleic Acid
Show More
Related Content
More details
DOI :
10.1016/0378-1119(91)90114-Q
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31120
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:59
Scientific Publication
Cloning and characterization of the Mycobacterium leprae putative ribosomal RNA promoter in Escherichia coli
98
Sela, S., Departments of Biology Washington University, St. Louis, MO 63130, United States
Clark-Curtiss, J.E., Departments of Biology Washington University, St. Louis, MO 63130, United States, Departments of Molecular Microbiology, Washington University, St. Louis, MO 63130, United States
Cloning and characterization of the Mycobacterium leprae putative ribosomal RNA promoter in Escherichia coli
The putative promoter region of the 16S ribosomal RNA-encoding gene (rRNA) of Mycobacterium leprae was cloned and characterized in Escherichia coli. A 932-bp HaeIII restriction fragment, containing the 5' end of the 16S rRNA gene and flanking upstream region, was cloned in front of a promoterless reporter gene in the shuttle vector, pMH109, to generate the plasmid, pYA1101. This clone exhibits promoter activity both in Gram- (E. coli) and Gram+ (Bacillus subtilis) bacteria. Sequence analysis and primer extension experiments with mRNA derived from the M. leprae clone were used to determine the structure and the location of the promoter, as well as the transcription start point in E. coli. The promoter region contains sequences that resemble the -35 and -10 consensus sequences found in many bacteria. A region located 34 bp distal to the promoter is a putative rRNA processing signal, based on sequence homology with processing signals involved in the maturation of the rRNA precursor in B. subtilis and several Mycoplasma species. © 1991.
Scientific Publication
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