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Development of a robust screening method for pathogenicity of Colletotric hum spp. on strawberry seedlings enabling forward genetic studies
Year:
2004
Source of publication :
Plant Disease
Authors :
Freeman, Stanley
;
.
Horowitz, Sigal Brown
;
.
Zveibil, Aida
;
.
Volume :
88
Co-Authors:
Horowitz, S., Dept. Plant Pathol. and Microbiol., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel, Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Yarden, O., Dept. Plant Pathol. and Microbiol., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Zveibil, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
845
To page:
851
(
Total pages:
7
)
Abstract:
Generation and screening for nonpathogenic mutants is a popular tool for identifying pathogenicity-related genes. Successful application of this technique for plant fungal pathosystems requires reliable and rapid screening procedures. This study reports on the development of a rapid in vitro bioassay enabling large-scale screening and isolation of nonpathogenic mutants of Colletotrichum gloeosporioides and C. acutatum on strawberry seedlings. Inoculation was carried out on strawberry seedlings at two different developmental stages: 12-week-old (young) and 15-week-old (older) seedlings. A comparison was made between two inoculation techniques, (i) foliar dip and (ii) root soak, at two incubation temperatures (19 and 25°C). Mortality of young seedlings was observed 4 days after inoculation with both species, reaching 50% within 10 days, using both techniques at 25°C. However, mortality of older seedlings was delayed by 4 days compared with that in the young seedlings when using the root-soak method. Disease development decreased in young and older seedlings at the lower temperature. This method also was reliable in determining pathogenicity of the cucurbit-specific C. magna that did not cause disease symptoms on strawberry by either inoculation method. The proposed method enabled screening of more than 980 restriction enzyme-mediated integration mutants resulting in a selection of five reduced-virulence isolates. Initial characterization of some of these mutants revealed large differences in germination and appressorial formation compared with pathogenic isolates.
Note:
Related Files :
Anthracnose
Colletotrichum
fungi
Genes
genetic engineering
germination
Inoculation
mutation
Seed
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31162
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:00
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Scientific Publication
Development of a robust screening method for pathogenicity of Colletotric hum spp. on strawberry seedlings enabling forward genetic studies
88
Horowitz, S., Dept. Plant Pathol. and Microbiol., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel, Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Yarden, O., Dept. Plant Pathol. and Microbiol., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Zveibil, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Development of a robust screening method for pathogenicity of Colletotric hum spp. on strawberry seedlings enabling forward genetic studies
Generation and screening for nonpathogenic mutants is a popular tool for identifying pathogenicity-related genes. Successful application of this technique for plant fungal pathosystems requires reliable and rapid screening procedures. This study reports on the development of a rapid in vitro bioassay enabling large-scale screening and isolation of nonpathogenic mutants of Colletotrichum gloeosporioides and C. acutatum on strawberry seedlings. Inoculation was carried out on strawberry seedlings at two different developmental stages: 12-week-old (young) and 15-week-old (older) seedlings. A comparison was made between two inoculation techniques, (i) foliar dip and (ii) root soak, at two incubation temperatures (19 and 25°C). Mortality of young seedlings was observed 4 days after inoculation with both species, reaching 50% within 10 days, using both techniques at 25°C. However, mortality of older seedlings was delayed by 4 days compared with that in the young seedlings when using the root-soak method. Disease development decreased in young and older seedlings at the lower temperature. This method also was reliable in determining pathogenicity of the cucurbit-specific C. magna that did not cause disease symptoms on strawberry by either inoculation method. The proposed method enabled screening of more than 980 restriction enzyme-mediated integration mutants resulting in a selection of five reduced-virulence isolates. Initial characterization of some of these mutants revealed large differences in germination and appressorial formation compared with pathogenic isolates.
Scientific Publication
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