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Priming of protein expression in the defence response of Zantedeschia aethiopica to Pectobacterium carotovorum
Year:
2014
Source of publication :
Molecular Plant Pathology
Authors :
Doron-Faigenboim, Adi
;
.
Yedidia, Iris
;
.
Volume :
15
Co-Authors:
Luzzatto-Knaan, T., Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel, Department of Plant Sciences, ARO, Volcani Center, Derech Hamacabim 20, PO Box 6, Bet Dagan, 50250, Israel
Kerem, Z., Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Doron-Faigenboim, A., Department of Plant Sciences, ARO, Volcani Center, Derech Hamacabim 20, PO Box 6, Bet Dagan, 50250, Israel
Yedidia, I., Department of Plant Sciences, ARO, Volcani Center, Derech Hamacabim 20, PO Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
364
To page:
378
(
Total pages:
15
)
Abstract:
The defence response of Zantedeschia aethiopica, a natural rhizomatous host of the soft rot bacterium Pectobacterium carotovorum, was studied following the activation of common induced resistance pathways-systemic acquired resistance and induced systemic resistance. Proteomic tools were used, together with invitro quantification and insitu localization of selected oxidizing enzymes. In total, 527 proteins were analysed by label-free mass spectrometry (MS) and annotated against the National Center for Biotechnology Information (NCBI) nonredundant (nr) protein database of rice (Oryza sativa). Of these, the fore most differentially expressed group comprised 215 proteins that were primed following application of methyl jasmonate (MJ) and subsequent infection with the pathogen. Sixty-five proteins were down-regulated following MJ treatments. The application of benzothiadiazole (BTH) increased the expression of 23 proteins; however, subsequent infection with the pathogen repressed their expression and did not induce priming. The sorting of primed proteins by Gene Ontology protein function category revealed that the primed proteins included nucleic acid-binding proteins, cofactor-binding proteins, ion-binding proteins, transferases, hydrolases and oxidoreductases. In line with the highlighted involvement of oxidoreductases in the defence response, we determined their activities, priming pattern and localization inplanta. Increased activities were confined to the area surrounding the pathogen penetration site, associating these enzymes with the induced systemic resistance afforded by the jasmonic acid signalling pathway. The results presented here demonstrate the concerted priming of protein expression following MJ treatment, making it a prominent part of the defence response of Z.aethiopica to P.carotovorum. © 2013 BSPP AND JOHN WILEY & SONS LTD.
Note:
Related Files :
Genetics
metabolism
Microbiology
Plant Disease
Plant Diseases
Zantedeschia
Show More
Related Content
More details
DOI :
10.1111/mpp.12100
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31183
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:00
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Scientific Publication
Priming of protein expression in the defence response of Zantedeschia aethiopica to Pectobacterium carotovorum
15
Luzzatto-Knaan, T., Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel, Department of Plant Sciences, ARO, Volcani Center, Derech Hamacabim 20, PO Box 6, Bet Dagan, 50250, Israel
Kerem, Z., Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Doron-Faigenboim, A., Department of Plant Sciences, ARO, Volcani Center, Derech Hamacabim 20, PO Box 6, Bet Dagan, 50250, Israel
Yedidia, I., Department of Plant Sciences, ARO, Volcani Center, Derech Hamacabim 20, PO Box 6, Bet Dagan, 50250, Israel
Priming of protein expression in the defence response of Zantedeschia aethiopica to Pectobacterium carotovorum
The defence response of Zantedeschia aethiopica, a natural rhizomatous host of the soft rot bacterium Pectobacterium carotovorum, was studied following the activation of common induced resistance pathways-systemic acquired resistance and induced systemic resistance. Proteomic tools were used, together with invitro quantification and insitu localization of selected oxidizing enzymes. In total, 527 proteins were analysed by label-free mass spectrometry (MS) and annotated against the National Center for Biotechnology Information (NCBI) nonredundant (nr) protein database of rice (Oryza sativa). Of these, the fore most differentially expressed group comprised 215 proteins that were primed following application of methyl jasmonate (MJ) and subsequent infection with the pathogen. Sixty-five proteins were down-regulated following MJ treatments. The application of benzothiadiazole (BTH) increased the expression of 23 proteins; however, subsequent infection with the pathogen repressed their expression and did not induce priming. The sorting of primed proteins by Gene Ontology protein function category revealed that the primed proteins included nucleic acid-binding proteins, cofactor-binding proteins, ion-binding proteins, transferases, hydrolases and oxidoreductases. In line with the highlighted involvement of oxidoreductases in the defence response, we determined their activities, priming pattern and localization inplanta. Increased activities were confined to the area surrounding the pathogen penetration site, associating these enzymes with the induced systemic resistance afforded by the jasmonic acid signalling pathway. The results presented here demonstrate the concerted priming of protein expression following MJ treatment, making it a prominent part of the defence response of Z.aethiopica to P.carotovorum. © 2013 BSPP AND JOHN WILEY & SONS LTD.
Scientific Publication
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