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Typing Prunus necrotic ringspot virus isolates by serology and restriction endonuclease analysis of PCR products
Year:
1999
Source of publication :
Annals of Applied Biology
Authors :
Maslenin, Ludmila
;
.
Rosner, Arie
;
.
Spiegel, Sara
;
.
Tam, Yehudit
;
.
Volume :
135
Co-Authors:
Spiegel, S., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Tam, Y., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Kolber, M., Plant Hlth. Soil Conserv. Stn. M., Budapest, Hungary
Nemeth, M., Plant Hlth. Soil Conserv. Stn. M., Budapest, Hungary
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Facilitators :
From page:
395
To page:
400
(
Total pages:
6
)
Abstract:
Prunus necrotic ringspot virus (PNRSV) isolates were characterised by bioassays, serotyping and restriction fragment length polymorphism (RFLP) analysis of PCR products. Based on symptoms in host trees and bioassays it was concluded that only one of the 16 tested isolates is severe. The serotyping results demonstrated that by using four different MAbs in TAS-ELISA the tested isolates could be divided into four subgroups, however, the severe isolate could not be singled out. RFLP analysis of PCR products supported the serotyping data but did not differentiate between isolates of the two main serological subgroups. A restriction map, derived from sequence analysis of the PCR products obtained from selected isolates, allowed exact location of the restriction sites within the PCR products of each isolate. A mild isolate with a unique genome structure was identified by both serological and RFLP assays. As far as we are aware, this is the first report on sub-grouping of PNRSV isolates by bioassays, serotyping with MAbs and RFLP analysis.
Note:
Related Files :
Bioassay
Ilarvirus
Polymerase Chain Reaction
Prunus
RFLP
Show More
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31201
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:00
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Scientific Publication
Typing Prunus necrotic ringspot virus isolates by serology and restriction endonuclease analysis of PCR products
135
Spiegel, S., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Tam, Y., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Kolber, M., Plant Hlth. Soil Conserv. Stn. M., Budapest, Hungary
Nemeth, M., Plant Hlth. Soil Conserv. Stn. M., Budapest, Hungary
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Typing Prunus necrotic ringspot virus isolates by serology and restriction endonuclease analysis of PCR products
Prunus necrotic ringspot virus (PNRSV) isolates were characterised by bioassays, serotyping and restriction fragment length polymorphism (RFLP) analysis of PCR products. Based on symptoms in host trees and bioassays it was concluded that only one of the 16 tested isolates is severe. The serotyping results demonstrated that by using four different MAbs in TAS-ELISA the tested isolates could be divided into four subgroups, however, the severe isolate could not be singled out. RFLP analysis of PCR products supported the serotyping data but did not differentiate between isolates of the two main serological subgroups. A restriction map, derived from sequence analysis of the PCR products obtained from selected isolates, allowed exact location of the restriction sites within the PCR products of each isolate. A mild isolate with a unique genome structure was identified by both serological and RFLP assays. As far as we are aware, this is the first report on sub-grouping of PNRSV isolates by bioassays, serotyping with MAbs and RFLP analysis.
Scientific Publication
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