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Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid biosynthesis
Year:
2011
Source of publication :
Planta
Authors :
Sadka, Avi
;
.
Shlizerman, Lyudmila A.
;
.
Zur, Naftali
;
.
Volume :
234
Co-Authors:
Degu, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Hatew, B., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Nunes-Nesi, A., Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
Shlizerman, L., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Zur, N., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Katz, E., Department of Plant Sciences, Mail Stop 5, University of California, 1 Shields Ave, Davis, CA 95616, United States
Fernie, A.R., Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
Blumwald, E., Department of Plant Sciences, Mail Stop 5, University of California, 1 Shields Ave, Davis, CA 95616, United States
Sadka, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
501
To page:
513
(
Total pages:
13
)
Abstract:
Citrate, a major determinant of citrus fruit quality, accumulates early in fruit development and declines towards maturation. The isomerization of citrate to isocitrate, catalyzed by aconitase is a key step in acid metabolism. Inhibition of mitochondrial aconitase activity early in fruit development contributes to acid accumulation, whereas increased cytosolic activity of aconitase causes citrate decline. It was previously hypothesized that the block in mitochondrial aconitase activity, inducing acid accumulation, is caused by citramalate. Here, we investigated the effect of citramalate and of another aconitase inhibitor, oxalomalate, on aconitase activity and regulation in callus originated from juice sacs. These compounds significantly increased citrate content and reduced the enzyme's activity, while slightly inducing its protein level. Citramalate inhibited the mitochondrial, but not cytosolic form of the enzyme. Its external application to mandarin fruits resulted in inhibition of aconitase activity, with a transient increase in fruit acidity detected a few weeks later. The endogenous level of citramalate was analyzed in five citrus varieties: its pattern of accumulation challenged the notion of its action as an endogenous inhibitor of mitochondrial aconitase. Metabolite profiling of oxalomalate-treated cells showed significant increases in a few amino acids and organic acids. The activities of alanine transaminase, aspartate transaminase and aspartate kinase, as well as these of two γ-aminobutyrate (GABA)-shunt enzymes, succinic semialdehyde reductase (SSAR) and succinic semialdehyde dehydrogenase (SSAD) were significantly induced in oxalomalate-treated cells. It is suggested that the increase in citrate, caused by aconitase inhibition, induces amino acid synthesis and the GABA shunt, in accordance with the suggested fate of citrate during the acid decline stage in citrus fruit. © 2011 Springer-Verlag.
Note:
Related Files :
Amino Acids
biosynthesis
Citramalate
drug effect
Genetics
Israel
metabolism
molecular genetics
oxalomalic acid
Show More
Related Content
More details
DOI :
10.1007/s00425-011-1411-2
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31233
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:00
Scientific Publication
Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid biosynthesis
234
Degu, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Hatew, B., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Nunes-Nesi, A., Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
Shlizerman, L., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Zur, N., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Katz, E., Department of Plant Sciences, Mail Stop 5, University of California, 1 Shields Ave, Davis, CA 95616, United States
Fernie, A.R., Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
Blumwald, E., Department of Plant Sciences, Mail Stop 5, University of California, 1 Shields Ave, Davis, CA 95616, United States
Sadka, A., Department of Fruit Tree Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid biosynthesis
Citrate, a major determinant of citrus fruit quality, accumulates early in fruit development and declines towards maturation. The isomerization of citrate to isocitrate, catalyzed by aconitase is a key step in acid metabolism. Inhibition of mitochondrial aconitase activity early in fruit development contributes to acid accumulation, whereas increased cytosolic activity of aconitase causes citrate decline. It was previously hypothesized that the block in mitochondrial aconitase activity, inducing acid accumulation, is caused by citramalate. Here, we investigated the effect of citramalate and of another aconitase inhibitor, oxalomalate, on aconitase activity and regulation in callus originated from juice sacs. These compounds significantly increased citrate content and reduced the enzyme's activity, while slightly inducing its protein level. Citramalate inhibited the mitochondrial, but not cytosolic form of the enzyme. Its external application to mandarin fruits resulted in inhibition of aconitase activity, with a transient increase in fruit acidity detected a few weeks later. The endogenous level of citramalate was analyzed in five citrus varieties: its pattern of accumulation challenged the notion of its action as an endogenous inhibitor of mitochondrial aconitase. Metabolite profiling of oxalomalate-treated cells showed significant increases in a few amino acids and organic acids. The activities of alanine transaminase, aspartate transaminase and aspartate kinase, as well as these of two γ-aminobutyrate (GABA)-shunt enzymes, succinic semialdehyde reductase (SSAR) and succinic semialdehyde dehydrogenase (SSAD) were significantly induced in oxalomalate-treated cells. It is suggested that the increase in citrate, caused by aconitase inhibition, induces amino acid synthesis and the GABA shunt, in accordance with the suggested fate of citrate during the acid decline stage in citrus fruit. © 2011 Springer-Verlag.
Scientific Publication
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