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An activating mutation of the Sclerotinia sclerotiorum pac1 gene increases oxalic acid production at low pH but decreases virulence
Year:
2007
Source of publication :
Molecular Plant Pathology
Authors :
Prusky, Dov
;
.
Volume :
8
Co-Authors:
Kim, Y.T., Department of Plant Pathology, University of Florida, 1453 Fifield Hall, PO Box 110680, Gainesville, FL 326110680, United States
Prusky, D., Volcani Center, Department of Postharvest Science of Fresh Produce, Bet Dagan, Israel
Rollins, J.A., Department of Plant Pathology, University of Florida, 1453 Fifield Hall, PO Box 110680, Gainesville, FL 326110680, United States
Facilitators :
From page:
611
To page:
622
(
Total pages:
12
)
Abstract:
The production of oxalic acid by Sclerotinia sclerotiorum is regulated by the ambient pH environment. This regulation and that of a few investigated pH-responsive genes is mediated in part by the zinc finger transcription factor encoded by pac1, an orthologue of the Aspergillus nidulans pacC gene. We manipulated the pac1 sequence by site-directed mutagenesis to create a dominant activating pac1c mutation and introduced this allele into a pac1 loss-of-function (Δpac1) strain. Consistent with a constitutive activation of Pac1 function, oxalic acid accumulation in recovered Δpac1-pac1 c strains was largely independent of ambient pH. Likewise, all three Δpac1-pac1c strains accumulated detectable pac1 transcripts in a pH 3 environment; however, accumulation of pac1 transcripts remained alkaline-inducible, but much reduced relative to wild-type in two of the three Δpac1-pac1c strains. Surprisingly, the accumulation of pg1 and acp1 transcripts, normally favoured by low pH conditions, were up-regulated across the range of ambient pH conditions examined (pH 3.4-7.2). Accumulation of neutral pH-expressed endopolygalacturonase-6 (pg6) transcripts, however, did not differ from wild-type. In pathogenicity assays using Arabidopsis and detached tomato leaflets, Δpac1-pac1c strains were reduced in virulence despite the ability to accumulate oxalic acid independent of the prevailing ambient pH environment. These results support the hypothesis that appropriate gene regulation in response to ambient pH is important for full S. sclerotiorum virulence independent of oxalic acid accumulation. © 2007 Blackwell publishing Ltd.
Note:
Related Files :
arabidopsis
Emericella nidulans
Lycopersicon esculentum
Sclerotinia sclerotiorum
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Related Content
More details
DOI :
10.1111/j.1364-3703.2007.00423.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31261
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
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Scientific Publication
An activating mutation of the Sclerotinia sclerotiorum pac1 gene increases oxalic acid production at low pH but decreases virulence
8
Kim, Y.T., Department of Plant Pathology, University of Florida, 1453 Fifield Hall, PO Box 110680, Gainesville, FL 326110680, United States
Prusky, D., Volcani Center, Department of Postharvest Science of Fresh Produce, Bet Dagan, Israel
Rollins, J.A., Department of Plant Pathology, University of Florida, 1453 Fifield Hall, PO Box 110680, Gainesville, FL 326110680, United States
An activating mutation of the Sclerotinia sclerotiorum pac1 gene increases oxalic acid production at low pH but decreases virulence
The production of oxalic acid by Sclerotinia sclerotiorum is regulated by the ambient pH environment. This regulation and that of a few investigated pH-responsive genes is mediated in part by the zinc finger transcription factor encoded by pac1, an orthologue of the Aspergillus nidulans pacC gene. We manipulated the pac1 sequence by site-directed mutagenesis to create a dominant activating pac1c mutation and introduced this allele into a pac1 loss-of-function (Δpac1) strain. Consistent with a constitutive activation of Pac1 function, oxalic acid accumulation in recovered Δpac1-pac1 c strains was largely independent of ambient pH. Likewise, all three Δpac1-pac1c strains accumulated detectable pac1 transcripts in a pH 3 environment; however, accumulation of pac1 transcripts remained alkaline-inducible, but much reduced relative to wild-type in two of the three Δpac1-pac1c strains. Surprisingly, the accumulation of pg1 and acp1 transcripts, normally favoured by low pH conditions, were up-regulated across the range of ambient pH conditions examined (pH 3.4-7.2). Accumulation of neutral pH-expressed endopolygalacturonase-6 (pg6) transcripts, however, did not differ from wild-type. In pathogenicity assays using Arabidopsis and detached tomato leaflets, Δpac1-pac1c strains were reduced in virulence despite the ability to accumulate oxalic acid independent of the prevailing ambient pH environment. These results support the hypothesis that appropriate gene regulation in response to ambient pH is important for full S. sclerotiorum virulence independent of oxalic acid accumulation. © 2007 Blackwell publishing Ltd.
Scientific Publication
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