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אסיף מאגר המחקר החקלאי
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Cloning and characterization of two citrus mitogen-activated protein kinase genes and effects of ethylene on their expression patterns
Year:
2000
Source of publication :
Acta Horticulturae
Authors :
Holland, Doron
;
.
Volume :
535
Co-Authors:
Zhong, G.Y., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Goren, R., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Riov, J., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Holland, D., Department of Fruit Tree Breeding and Molecular Genetics, Institute of Horticulture, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
107
To page:
111
(
Total pages:
5
)
Abstract:
The influence of the simple gas ethylene on the activity of a Citrus myelin basic protein kinase was investigated. Continuous ethylene treatment was found to activate a protein kinase. This kinase also seemed to be induced by wounding. Full-length cDNAs of two Citrus mitogen-activated protein kinase genes (CMAPK1 and CMAPK2) were isolated from a Citrus cDNA library. Their recombinant proteins produced in bacteria were recognized by anti-human ERK antibodies, and the in-gel kinase activity assay showed that CMAPK2 recombinant protein was capable of autophosphorylation. Northern blot analysis showed that ethylene induced both genes transcriptionally. CMAPK2 was also induced by wounding treatment, whereas CMAPK1 was not. In situ hybridization revealed that both of the MAPK genes had unusual transcript distribution patterns across the fruit abscission zone.
Note:
Related Files :
Bacteria (microorganisms)
Citrus
ethylene
Mitogen-activated protein kinase
Wounding
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
31275
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
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Scientific Publication
Cloning and characterization of two citrus mitogen-activated protein kinase genes and effects of ethylene on their expression patterns
535
Zhong, G.Y., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Goren, R., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Riov, J., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Holland, D., Department of Fruit Tree Breeding and Molecular Genetics, Institute of Horticulture, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Cloning and characterization of two citrus mitogen-activated protein kinase genes and effects of ethylene on their expression patterns
The influence of the simple gas ethylene on the activity of a Citrus myelin basic protein kinase was investigated. Continuous ethylene treatment was found to activate a protein kinase. This kinase also seemed to be induced by wounding. Full-length cDNAs of two Citrus mitogen-activated protein kinase genes (CMAPK1 and CMAPK2) were isolated from a Citrus cDNA library. Their recombinant proteins produced in bacteria were recognized by anti-human ERK antibodies, and the in-gel kinase activity assay showed that CMAPK2 recombinant protein was capable of autophosphorylation. Northern blot analysis showed that ethylene induced both genes transcriptionally. CMAPK2 was also induced by wounding treatment, whereas CMAPK1 was not. In situ hybridization revealed that both of the MAPK genes had unusual transcript distribution patterns across the fruit abscission zone.
Scientific Publication
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