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Detection wavelength and integration strategy for amino acid analysis
Year:
1986
Source of publication :
Journal of Chromatography A
Authors :
Harduf, Zvi
;
.
Volume :
363
Co-Authors:
Harduf, Z., Agricultural Research Organisation, The Volcani Center, Department of Animal Nutrition, P.O. Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
261
To page:
266
(
Total pages:
6
)
Abstract:
Amino as well as imino acids were detected with ninhydrin at a single wavelength of 405 nm, one of the absorption maxima of Ruhemann's purple, instead of the conventional 570 and 440 nm. The advantages are: (1) no need for a wavelength switch before and after the imino acid peaks; (2) avoids summing of the signals of 40 and 570 nm; (3) improves the sensitivity for most of the amino acids, particularly glutamic, cystine, methionine and lysine. The increase in the wavelength bandwidth, in order to increase the signal-to-noise ratio, is somewhat limited compared with that at 570 nm. © 1986.
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DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31316
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
Scientific Publication
Detection wavelength and integration strategy for amino acid analysis
363
Harduf, Z., Agricultural Research Organisation, The Volcani Center, Department of Animal Nutrition, P.O. Box 6, Bet Dagan, 50250, Israel
Detection wavelength and integration strategy for amino acid analysis
Amino as well as imino acids were detected with ninhydrin at a single wavelength of 405 nm, one of the absorption maxima of Ruhemann's purple, instead of the conventional 570 and 440 nm. The advantages are: (1) no need for a wavelength switch before and after the imino acid peaks; (2) avoids summing of the signals of 40 and 570 nm; (3) improves the sensitivity for most of the amino acids, particularly glutamic, cystine, methionine and lysine. The increase in the wavelength bandwidth, in order to increase the signal-to-noise ratio, is somewhat limited compared with that at 570 nm. © 1986.
Scientific Publication
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