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The elicitation of ethylene biosynthesis by a Trichoderma xylanase is not related to the cell wall degradation activity of the enzyme
Year:
1993
Source of publication :
Plant physiology (source)
Authors :
Fuchs, Yoram
;
.
Volume :
102
Co-Authors:
Sharon, A., Department of Horticulture, University of Maryland, Beltsville, MD 20705, United States, Department of Plant Pathology, 334 Plant Science Building, Cornell University, Ithaca, NY 14853-5908, United States
Fuchs, Y., Hort. Crops Quality Laboratory, Beltsville Agric. Res. Center West, U. States Department of Agriculture, Beltsville, MD 20705, United States, Dept. of Fruit and Vegetable Storage, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Anderson, J.D., Weed Science Laboratory, Beltsville Agric. Res. Center West, U. States Department of Agriculture, Beltsville, MD 20705, United States
Facilitators :
From page:
1325
To page:
1329
(
Total pages:
5
)
Abstract:
A β-1,4-endoxylanase (EIX) isolated from Trichoderma viride elicits plant defense responses in certain tobacco (Nicotiana tabacum L.) cultivars in addition to its xylan degradation activity. It was not clear whether elicitation occurs by cell wall fragments released by the enzymic activity or by the xylanase protein interacting directly with the plant cells. We used protoplasts isolated from tobacco leaves to test whether the cell wall is required for the stimulation of ethylene biosynthesis by EIX. Protoplasts of tobacco (cv Xanthi) responded to treatment with the EIX, as indicated by an increased production of ethylene and the loss of protoplast viability. Protoplasts prepared from ethylene-pretreated leaves produced more ethylene and had higher rates of cell death in response to EIX than protoplasts prepared from nonethylene-treated leaves. Protoplasts of an EIX-insensitive cultivar of tobacco (Hicks) were insensitive to high concentrations of EIX. The addition of a crude cell wall preparation to protoplasts during incubation with EIX did not enhance the induction of ethylene biosynthesis by nonsaturating as well as saturating concentrations of EIX. These data indicate that the xylanase activity of EIX is unrelated to the elicitation of ethylene biosynthesis through the production of some cell wall fragment, since the protein per se appears capable of eliciting ethylene biosynthesis in protoplasts.
Note:
Related Files :
Nicotiana tabacum
Tabacum
Trichoderma
Trichoderma viride
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Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31333
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
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Scientific Publication
The elicitation of ethylene biosynthesis by a Trichoderma xylanase is not related to the cell wall degradation activity of the enzyme
102
Sharon, A., Department of Horticulture, University of Maryland, Beltsville, MD 20705, United States, Department of Plant Pathology, 334 Plant Science Building, Cornell University, Ithaca, NY 14853-5908, United States
Fuchs, Y., Hort. Crops Quality Laboratory, Beltsville Agric. Res. Center West, U. States Department of Agriculture, Beltsville, MD 20705, United States, Dept. of Fruit and Vegetable Storage, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Anderson, J.D., Weed Science Laboratory, Beltsville Agric. Res. Center West, U. States Department of Agriculture, Beltsville, MD 20705, United States
The elicitation of ethylene biosynthesis by a Trichoderma xylanase is not related to the cell wall degradation activity of the enzyme
A β-1,4-endoxylanase (EIX) isolated from Trichoderma viride elicits plant defense responses in certain tobacco (Nicotiana tabacum L.) cultivars in addition to its xylan degradation activity. It was not clear whether elicitation occurs by cell wall fragments released by the enzymic activity or by the xylanase protein interacting directly with the plant cells. We used protoplasts isolated from tobacco leaves to test whether the cell wall is required for the stimulation of ethylene biosynthesis by EIX. Protoplasts of tobacco (cv Xanthi) responded to treatment with the EIX, as indicated by an increased production of ethylene and the loss of protoplast viability. Protoplasts prepared from ethylene-pretreated leaves produced more ethylene and had higher rates of cell death in response to EIX than protoplasts prepared from nonethylene-treated leaves. Protoplasts of an EIX-insensitive cultivar of tobacco (Hicks) were insensitive to high concentrations of EIX. The addition of a crude cell wall preparation to protoplasts during incubation with EIX did not enhance the induction of ethylene biosynthesis by nonsaturating as well as saturating concentrations of EIX. These data indicate that the xylanase activity of EIX is unrelated to the elicitation of ethylene biosynthesis through the production of some cell wall fragment, since the protein per se appears capable of eliciting ethylene biosynthesis in protoplasts.
Scientific Publication
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