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Changes in ascorbic acid concentration, ascorbate oxidase activity, and apoplastic pH in relation to fruit development in pepper (Capsicum annuum L.) and the occurrence of blossom-end rot
Year:
2008
Authors :
Aloni, Benyamin
;
.
Deventurero, Gila
;
.
Karni, Leah
;
.
Volume :
83
Co-Authors:
Aloni, B., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel
Karni, L., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel
Deventurero, G., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel
Turhan, E., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel, Canakkale Onsekiz Mart University, Bayramic Vocational College, 17700 Bayramic, Canakkale, Turkey
Aktas, H., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel, Harran University, Faculty of Agriculture, Department of Horticulture, 63040 Sanliurfa, Turkey
Facilitators :
From page:
100
To page:
105
(
Total pages:
6
)
Abstract:
We recently suggested that the occurrence of ascorbic acid (AsA) in the apoplast of bell pepper fruit may be part of the protection mechanism against blossom-end rot (BER). In the present study, we determined AsA concentrations, changes in pH, and the activities of ascorbate oxidase (AO) in the apoplast and symplast of pepper fruit, in relation to fruit development and the occurrence of BER. In healthy pepper fruit, the concentrations of AsA in the symplast and apoplast increased during fruit development, whereas the activity of AO decreased when measured at the pH typical of each developmental stage. The apoplastic pH decreased from 6.2 in young fruit to 4.8 in mature red fruit; while the symplastic pH did not change during fruit development. Following high-temperature treatment (35°C for 48 h in the dark), the concentration of apoplastic AsA, and the activity of galacturonic acid reductase (which participates in the AsA biosynthetic pathway), decreased, while there was an increase in apoplastic pH. The AsA content decreased in the apoplast of BER-affected fruit, and the pH was more alkaline (pH 6.9) than in healthy fruit (pH 6.0). In young fruit with BER, the apoplastic AO activity increased and the symplastic galacturonic acid reductase activity was inhibited. We suggest that a reduction in apoplastic AsA concentration, an increase in apoplastic AO activity, and changes in apoplastic pH in fruit prone to BER may be part of the mechanism which leads to the occurrence of BER symptoms. Whether or not there are causal relationships between these changes is not yet clear.
Note:
Related Files :
Capsicum annuum
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DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31365
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
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Scientific Publication
Changes in ascorbic acid concentration, ascorbate oxidase activity, and apoplastic pH in relation to fruit development in pepper (Capsicum annuum L.) and the occurrence of blossom-end rot
83
Aloni, B., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel
Karni, L., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel
Deventurero, G., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel
Turhan, E., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel, Canakkale Onsekiz Mart University, Bayramic Vocational College, 17700 Bayramic, Canakkale, Turkey
Aktas, H., Department of Vegetable Crop Research, Agricultural Research Organisation, Volcani Center, Bet Dagan 50250, Israel, Harran University, Faculty of Agriculture, Department of Horticulture, 63040 Sanliurfa, Turkey
Changes in ascorbic acid concentration, ascorbate oxidase activity, and apoplastic pH in relation to fruit development in pepper (Capsicum annuum L.) and the occurrence of blossom-end rot
We recently suggested that the occurrence of ascorbic acid (AsA) in the apoplast of bell pepper fruit may be part of the protection mechanism against blossom-end rot (BER). In the present study, we determined AsA concentrations, changes in pH, and the activities of ascorbate oxidase (AO) in the apoplast and symplast of pepper fruit, in relation to fruit development and the occurrence of BER. In healthy pepper fruit, the concentrations of AsA in the symplast and apoplast increased during fruit development, whereas the activity of AO decreased when measured at the pH typical of each developmental stage. The apoplastic pH decreased from 6.2 in young fruit to 4.8 in mature red fruit; while the symplastic pH did not change during fruit development. Following high-temperature treatment (35°C for 48 h in the dark), the concentration of apoplastic AsA, and the activity of galacturonic acid reductase (which participates in the AsA biosynthetic pathway), decreased, while there was an increase in apoplastic pH. The AsA content decreased in the apoplast of BER-affected fruit, and the pH was more alkaline (pH 6.9) than in healthy fruit (pH 6.0). In young fruit with BER, the apoplastic AO activity increased and the symplastic galacturonic acid reductase activity was inhibited. We suggest that a reduction in apoplastic AsA concentration, an increase in apoplastic AO activity, and changes in apoplastic pH in fruit prone to BER may be part of the mechanism which leads to the occurrence of BER symptoms. Whether or not there are causal relationships between these changes is not yet clear.
Scientific Publication
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