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DNAase I sensitivity of genes expressed during myogenesis
Year:
1982
Source of publication :
Nucleic Acids Research
Authors :
Shani, Moshe
;
.
Volume :
10
Co-Authors:
Carmon, Y., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Czosnek, H., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Nudel, U., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Shani, M., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Yaffe, D., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
3085
To page:
3097
(
Total pages:
13
)
Abstract:
Cultures of a rat myogenic cell line were used to examine the question of whether in proliferating precursor cells genes which are programmed to be expressed later in development, in the same cell lineage, differ in DNAase I sensitivity from genes which are never expressed in these cells. Nuclei isolated from proliferating mononucleated myoblasts, differentiated cultures containing multinucleaged fibers, and rat brain, were treated with DNAase I. The sensitivity of the genes coding for the muscle-specific α-actin, myosin light chain 2 and the nonmuscle β-actin was measured by blot hybridization of nuclear DNA with the corresponding cloned cDNA and genomic DNA probes. The sensitivity of these genes was compared to that of a gene not expressed in the muscle tissue. The results showed that in the muscle precursor cells, the potentiality of tissue-specific genes to be expressed is not reflected in DNAase I sensitivity. The changes which render these genes preferentially sensitive to DNAase I take place during the transition to terminal differentiation. The results showed also that the region of DNAase I sensitivity of the α-actin gene in the differentiated cells ends between 40 to 700 bp 5' to the structural gene. No DNAase I hypersensitive site was detected 5' to the αactin gene. © 1982 IRL Press Limited.
Note:
Related Files :
Animal
Cell Division
cell line
Deoxyribonuclease I
drug effect
Genetics
Kinetics
metabolism
myosin
structural gene
Show More
Related Content
More details
DOI :
10.1093/nar/10.10.3085
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31375
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
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Scientific Publication
DNAase I sensitivity of genes expressed during myogenesis
10
Carmon, Y., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Czosnek, H., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Nudel, U., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Shani, M., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Yaffe, D., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
DNAase I sensitivity of genes expressed during myogenesis
Cultures of a rat myogenic cell line were used to examine the question of whether in proliferating precursor cells genes which are programmed to be expressed later in development, in the same cell lineage, differ in DNAase I sensitivity from genes which are never expressed in these cells. Nuclei isolated from proliferating mononucleated myoblasts, differentiated cultures containing multinucleaged fibers, and rat brain, were treated with DNAase I. The sensitivity of the genes coding for the muscle-specific α-actin, myosin light chain 2 and the nonmuscle β-actin was measured by blot hybridization of nuclear DNA with the corresponding cloned cDNA and genomic DNA probes. The sensitivity of these genes was compared to that of a gene not expressed in the muscle tissue. The results showed that in the muscle precursor cells, the potentiality of tissue-specific genes to be expressed is not reflected in DNAase I sensitivity. The changes which render these genes preferentially sensitive to DNAase I take place during the transition to terminal differentiation. The results showed also that the region of DNAase I sensitivity of the α-actin gene in the differentiated cells ends between 40 to 700 bp 5' to the structural gene. No DNAase I hypersensitive site was detected 5' to the αactin gene. © 1982 IRL Press Limited.
Scientific Publication
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