נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Quantitative and qualitative analysis of Botrytis inoculated on table grapes by qPCR and antibodies
Year:
2009
Source of publication :
Postharvest Biology and Technology
Authors :
Ish-Shalom, Shahar
;
.
Kaplunov, Tatiana
;
.
Lichter, Amnon
;
.
Lurie, Susan
;
.
Zutahy, Yohanan
;
.
Volume :
52
Co-Authors:
Celik, M., Adnan Menderes University, Agriculture Faculty, Horticulture Department, Aydin, 09100, Turkey
Kalpulov, T., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Zutahy, Y., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Ish-shalom, S., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Lurie, S., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Lichter, A., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
235
To page:
239
(
Total pages:
5
)
Abstract:
The fungus Botrytis cinerea is the major cause of decay in table grapes during storage, and the severity of decay depends in part on contamination with the fungus before storage. The current SO2 technology to prevent decay is robust and independent of the level of contamination by B. cinerea. The introduction of alternative technologies may however require implementation of means which are proportional to the level of contamination. The objectives of this study were to test the feasibility of quantifying B. cinerea in artificially inoculated grapes and to monitor the progress of disease during storage. Two methods were compared for detection of B. cinerea in grapes; an antibody kit specific for B. cinerea, and quantitative PCR using fungal specific primers. Antibodies for fast detection of B. cinerea yielded positive results only in the later stages of decay development. In contrast, the quantitative PCR demonstrated positive identification of the fungus at all storage time points, and found increasing amounts of the fungus during storage. © 2008 Elsevier B.V. All rights reserved.
Note:
Related Files :
Antibody test
Botrytis
Disease monitoring
fungi
Real-time PCR
Storage
Table grapes
Vitaceae
Show More
Related Content
More details
DOI :
10.1016/j.postharvbio.2008.10.007
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31382
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
Scientific Publication
Quantitative and qualitative analysis of Botrytis inoculated on table grapes by qPCR and antibodies
52
Celik, M., Adnan Menderes University, Agriculture Faculty, Horticulture Department, Aydin, 09100, Turkey
Kalpulov, T., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Zutahy, Y., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Ish-shalom, S., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Lurie, S., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Lichter, A., Department of Postharvest Science, ARO, The Volcani Center, POB 6, Bet Dagan, 50250, Israel
Quantitative and qualitative analysis of Botrytis inoculated on table grapes by qPCR and antibodies
The fungus Botrytis cinerea is the major cause of decay in table grapes during storage, and the severity of decay depends in part on contamination with the fungus before storage. The current SO2 technology to prevent decay is robust and independent of the level of contamination by B. cinerea. The introduction of alternative technologies may however require implementation of means which are proportional to the level of contamination. The objectives of this study were to test the feasibility of quantifying B. cinerea in artificially inoculated grapes and to monitor the progress of disease during storage. Two methods were compared for detection of B. cinerea in grapes; an antibody kit specific for B. cinerea, and quantitative PCR using fungal specific primers. Antibodies for fast detection of B. cinerea yielded positive results only in the later stages of decay development. In contrast, the quantitative PCR demonstrated positive identification of the fungus at all storage time points, and found increasing amounts of the fungus during storage. © 2008 Elsevier B.V. All rights reserved.
Scientific Publication
You may also be interested in