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Four linked genes participate in controlling sporulation efficiency in budding yeast
Year:
2006
Source of publication :
PLoS genetics
Authors :
Ben-Ari, Giora
;
.
Lavi, Uri
;
.
Sherman, Amir
;
.
Volume :
2
Co-Authors:
Ben-Ari, G., Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ont., Canada
Zenvirth, D., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Sherman, A., Agricultural Research Organization, Beit Dagan, Israel
David, L., Stanford Genome Technology Center, Palo Alto, CA, United States, Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, United States
Klutstein, M., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Lavi, U., Agricultural Research Organization, Beit Dagan, Israel
Hillel, J., Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Simchen, G., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Facilitators :
From page:
1815
To page:
1823
(
Total pages:
9
)
Abstract:
Quantitative traits are conditioned by several genetic determinants. Since such genes influence many important complex traits in various organisms, the identification of quantitative trait loci (QTLs) is of major interest, but still encounters serious difficulties. We detected four linked genes within one QTL, which participate in controlling sporulation efficiency in Saccharomyces cerevisiae. Following the identification of single nucleotide polymorphisms by comparing the sequences of 145 genes between the parental strains SK1 and S288c, we analyzed the segregating progeny of the cross between them. Through reciprocal hemizygosity analysis, four genes, RAS2, PMS1, SWS2, and FKH2, located in a region of 60 kilobases on Chromosome 14, were found to be associated with sporulation efficiency. Three of the four "high" sporulation alleles are derived from the "low" sporulating strain. Two of these sporulationrelated genes were verified through allele replacements. For RAS2, the causative variation was suggested to be a single nucleotide difference in the upstream region of the gene. This quantitative trait nucleotide accounts for sporulation variability among a set of ten closely related winery yeast strains. Our results provide a detailed view of genetic complexity in one "QTL region" that controls a quantitative trait and reports a single nucleotide polymorphism-trait association in wild strains. Moreover, these findings have implications on QTL identification in higher eukaryotes. © 2006 Ben-Ari et al.
Note:
Related Files :
Eukaryota
gene sequence
genetic linkage
quantitative trait
quantitative trait locus
sporogenesis
sws2 gene
Yeast
Show More
Related Content
More details
DOI :
10.1371/journal.pgen.0020195
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31392
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
Scientific Publication
Four linked genes participate in controlling sporulation efficiency in budding yeast
2
Ben-Ari, G., Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ont., Canada
Zenvirth, D., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Sherman, A., Agricultural Research Organization, Beit Dagan, Israel
David, L., Stanford Genome Technology Center, Palo Alto, CA, United States, Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, United States
Klutstein, M., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Lavi, U., Agricultural Research Organization, Beit Dagan, Israel
Hillel, J., Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Simchen, G., Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
Four linked genes participate in controlling sporulation efficiency in budding yeast
Quantitative traits are conditioned by several genetic determinants. Since such genes influence many important complex traits in various organisms, the identification of quantitative trait loci (QTLs) is of major interest, but still encounters serious difficulties. We detected four linked genes within one QTL, which participate in controlling sporulation efficiency in Saccharomyces cerevisiae. Following the identification of single nucleotide polymorphisms by comparing the sequences of 145 genes between the parental strains SK1 and S288c, we analyzed the segregating progeny of the cross between them. Through reciprocal hemizygosity analysis, four genes, RAS2, PMS1, SWS2, and FKH2, located in a region of 60 kilobases on Chromosome 14, were found to be associated with sporulation efficiency. Three of the four "high" sporulation alleles are derived from the "low" sporulating strain. Two of these sporulationrelated genes were verified through allele replacements. For RAS2, the causative variation was suggested to be a single nucleotide difference in the upstream region of the gene. This quantitative trait nucleotide accounts for sporulation variability among a set of ten closely related winery yeast strains. Our results provide a detailed view of genetic complexity in one "QTL region" that controls a quantitative trait and reports a single nucleotide polymorphism-trait association in wild strains. Moreover, these findings have implications on QTL identification in higher eukaryotes. © 2006 Ben-Ari et al.
Scientific Publication
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