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Generation of growth hormone binding protein by avian growth plate chondrocytes is dependent on cell differentiation
Year:
1997
Authors :
Hurvitz, Shmuel (Animal science)
;
.
Lavelin, Irina
;
.
Monsonego-Ornan, Efrat
;
.
Pines, Mark
;
.
Volume :
135
Co-Authors:
Monsonego, E., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Baumbach, W.R., Molecular and Cellular Biology Group, Agric. Res. Div., Amer. Cyanamid Co., Princeton, NJ 08543, United States
Lavelin, I., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Gertler, A., Institute of Biochemistry, Food Sci. Hum. Nutr., Hebrew U., Rehovot 76100, Israel
Hurwitz, S., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Pines, M., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
1
To page:
10
(
Total pages:
10
)
Abstract:
Growth hormone receptor (GH-R) gene expression was evaluated in avian growth-plates in situ and in cultured chondrocytes. In the epiphyseal growth-plate, chondrocytes at different stages of differentiation located at the proliferative and upper hypertrophic zones express the GH-R gene. In culture, addition of ascorbic acid facilitated chondrocyte differentiation as evaluated by decrease in collagen type II gene expression and increase in alkaline phosphatase activity and osteopontin gene expression. Both the ascorbic acid-treated and untreated chondrocytes expressed the gene coding for the chicken growth hormone receptor (cGH-R), but only the undifferentiated cells were capable of binding the hormone. This reduction in GH-binding resulted in alteration in GH-dependent regulation of the GH-R gene expression: only the undifferentiated chondrocytes responded to chicken GH (cGH) by down-regulation of the cGH-R gene expression. Chondrocyte differentiation induced by either ascorbic acid or retinoic acid was associated with the appearance of two growth hormone binding-proteins (GHBPs) in the culture medium with estimated MWs of 32 and 70 kDa, respectively. These GHBPs differ in their MW from the major GHBP found in chicken plasma. Chondrocyte GHBPs specifically bind [125I]cGH, which can be displaced by an excess of unlabeled cGH. The differentiation-dependent increase in the 70 kDa GHBP was observed also using specific chicken GHBP antiserum. Our data suggest that the reduction of the differentiated chondrocytes response to GH is due to differentiation-dependent loss of the extracellular domain of the GH-R, resulting in a lack of functional receptors on the cell surface and generation of GHBP.
Note:
Related Files :
animal cell
Animals
Chickens
fowl
gene expression
Growth plate
hypertrophy
Receptors, Somatotropin
Show More
Related Content
More details
DOI :
10.1016/S0303-7207(97)00154-8
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31404
Last updated date:
21/08/2022 07:45
Creation date:
17/04/2018 01:02
Scientific Publication
Generation of growth hormone binding protein by avian growth plate chondrocytes is dependent on cell differentiation
135
Monsonego, E., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Baumbach, W.R., Molecular and Cellular Biology Group, Agric. Res. Div., Amer. Cyanamid Co., Princeton, NJ 08543, United States
Lavelin, I., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Gertler, A., Institute of Biochemistry, Food Sci. Hum. Nutr., Hebrew U., Rehovot 76100, Israel
Hurwitz, S., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Pines, M., Institute of Animal Science, Agric. Res. Org., the Volcani Center, Bet Dagan 50250, Israel
Generation of growth hormone binding protein by avian growth plate chondrocytes is dependent on cell differentiation
Growth hormone receptor (GH-R) gene expression was evaluated in avian growth-plates in situ and in cultured chondrocytes. In the epiphyseal growth-plate, chondrocytes at different stages of differentiation located at the proliferative and upper hypertrophic zones express the GH-R gene. In culture, addition of ascorbic acid facilitated chondrocyte differentiation as evaluated by decrease in collagen type II gene expression and increase in alkaline phosphatase activity and osteopontin gene expression. Both the ascorbic acid-treated and untreated chondrocytes expressed the gene coding for the chicken growth hormone receptor (cGH-R), but only the undifferentiated cells were capable of binding the hormone. This reduction in GH-binding resulted in alteration in GH-dependent regulation of the GH-R gene expression: only the undifferentiated chondrocytes responded to chicken GH (cGH) by down-regulation of the cGH-R gene expression. Chondrocyte differentiation induced by either ascorbic acid or retinoic acid was associated with the appearance of two growth hormone binding-proteins (GHBPs) in the culture medium with estimated MWs of 32 and 70 kDa, respectively. These GHBPs differ in their MW from the major GHBP found in chicken plasma. Chondrocyte GHBPs specifically bind [125I]cGH, which can be displaced by an excess of unlabeled cGH. The differentiation-dependent increase in the 70 kDa GHBP was observed also using specific chicken GHBP antiserum. Our data suggest that the reduction of the differentiated chondrocytes response to GH is due to differentiation-dependent loss of the extracellular domain of the GH-R, resulting in a lack of functional receptors on the cell surface and generation of GHBP.
Scientific Publication
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