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Mayer, R.T., Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, College Station, TX 77840, United States
Durrant, J.L., Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, College Station, TX 77840, United States
Holman, G.M., Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, College Station, TX 77840, United States
Weirich, G.F., Insect Physiology Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, Beltsville, MD 20705, United States
Svoboda, J.A., Insect Physiology Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, Beltsville, MD 20705, United States
Ecdysone 3-epimerase was partially purified by ammonium sulfate fractionation from the 100,000 g supernate of Manduca sexta midguts. The enzyme converts ecdysone and 20-hydroxyecdysone to their respective 3-epimers, requires NADH or NADPH and 02 for this reaction, and has the following kinetic parameters: for ecdysone, Km = 17.0 ± 1.4 μM, Vmax = 110.6 ± 14.6 pmol min-1 mg-1; for 20-hydroxyecdysone, Km = 47.3 ± 7.5 μM, Vmax = 131.0 ± 3.5 pmol min-1 mg-1: for NADPH, Km = 85.4 ± 10.6 μM; for NADH, Km = 51.3 ± 1.3 μM. The reaction is irreversible and can be inhibited by various ecdysteroids. © 1979 Holden-Day Inc.
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Ecdysone 3-epimerase from the midgut of Manduca sexta (L.)
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Mayer, R.T., Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, College Station, TX 77840, United States
Durrant, J.L., Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, College Station, TX 77840, United States
Holman, G.M., Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, College Station, TX 77840, United States
Weirich, G.F., Insect Physiology Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, Beltsville, MD 20705, United States
Svoboda, J.A., Insect Physiology Laboratory, Agricultural Research-Science and Education Administration, U. S. Department of Agriculture, Beltsville, MD 20705, United States
Ecdysone 3-epimerase from the midgut of Manduca sexta (L.)
Ecdysone 3-epimerase was partially purified by ammonium sulfate fractionation from the 100,000 g supernate of Manduca sexta midguts. The enzyme converts ecdysone and 20-hydroxyecdysone to their respective 3-epimers, requires NADH or NADPH and 02 for this reaction, and has the following kinetic parameters: for ecdysone, Km = 17.0 ± 1.4 μM, Vmax = 110.6 ± 14.6 pmol min-1 mg-1; for 20-hydroxyecdysone, Km = 47.3 ± 7.5 μM, Vmax = 131.0 ± 3.5 pmol min-1 mg-1: for NADPH, Km = 85.4 ± 10.6 μM; for NADH, Km = 51.3 ± 1.3 μM. The reaction is irreversible and can be inhibited by various ecdysteroids. © 1979 Holden-Day Inc.
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