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אסיף מאגר המחקר החקלאי
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Analysis of myogenesis with recombinant DNA techniques.
Year:
1982
Authors :
Shani, Moshe
;
.
Volume :
158
Co-Authors:
Yaffe, D.
Nudel, U.
Czosnek, H.
Zakut, R.
Carmon, Y.
Shani, M.
Facilitators :
From page:
127
To page:
137
(
Total pages:
11
)
Abstract:
Recombinant phages containing the rat skeletal muscle alpha-actin gene and the cytoplasmic beta-actin gene were isolated and the structure of these genes was determined. Both genes contain a large intron in the 5' untranslated region and smaller introns at codons 41, 267 and 327. In addition, the alpha-actin contains introns at codons 150 and 204 not present in the beta-actin gene, whereas the beta-actin gene contains an intron at codon 121. The evolutionary aspects of these findings are discussed. Active genes are organized in chromatin in a conformation which renders them preferentially sensitive to digestion with nucleolytic enzymes. The DNAase I sensitivity of genes programmed to be expressed during myogenesis was tested in a cloned cell population of a myogenic cell line. It was found that these genes are not preferentially sensitive to DNAase I in the chromatin of proliferating mononucleated cells. They become DNAase I sensitive during terminal differentiation.
Note:
Related Files :
Animal
Base Sequence
Deoxyribonuclease I
Genetics
metabolism
muscle
Muscles
structural gene
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31429
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
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Scientific Publication
Analysis of myogenesis with recombinant DNA techniques.
158
Yaffe, D.
Nudel, U.
Czosnek, H.
Zakut, R.
Carmon, Y.
Shani, M.
Analysis of myogenesis with recombinant DNA techniques.
Recombinant phages containing the rat skeletal muscle alpha-actin gene and the cytoplasmic beta-actin gene were isolated and the structure of these genes was determined. Both genes contain a large intron in the 5' untranslated region and smaller introns at codons 41, 267 and 327. In addition, the alpha-actin contains introns at codons 150 and 204 not present in the beta-actin gene, whereas the beta-actin gene contains an intron at codon 121. The evolutionary aspects of these findings are discussed. Active genes are organized in chromatin in a conformation which renders them preferentially sensitive to digestion with nucleolytic enzymes. The DNAase I sensitivity of genes programmed to be expressed during myogenesis was tested in a cloned cell population of a myogenic cell line. It was found that these genes are not preferentially sensitive to DNAase I in the chromatin of proliferating mononucleated cells. They become DNAase I sensitive during terminal differentiation.
Scientific Publication
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