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Glyphosate-induced anther indehiscence in cotton is partially temperature dependent and involves cytoskeleton and secondary wall modifications and auxin accumulation
Year:
2006
Source of publication :
Plant physiology (source)
Authors :
Abu-Abied, Mohamad
;
.
Belausov, Eduard
;
.
Sadot, Einat
;
.
Yasuor, Hagai
;
.
Volume :
141
Co-Authors:
Yasuor, H., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Abu-Abied, M., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Madmony, A., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sadot, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Riov, J., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Rubin, B., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Facilitators :
From page:
1306
To page:
1315
(
Total pages:
10
)
Abstract:
Yield reduction caused by late application of glyphosate to glyphosate-resistant cotton (Gossypium hirsutum; GRC) expressing CP4 5-enol-pyruvylshikmate-3-P synthase under the cauliflower mosaic virus-35S promoter has been attributed to male sterility. This study was aimed to elucidate the factors and mechanisms involved in this phenomenon. Western and tissue-print blots demonstrated a reduced expression of the transgene in anthers of GRC compared to ovules of the same plants. Glyphosate application to GRC grown at a high temperature regime after the initiation of flower buds caused a complete loss of pollen viability and inhibition of anther dehiscence, while at a moderate temperature regime only 50% of the pollen grains were disrupted and anther dehiscence was normal. Glyphosate-damaged anthers exhibited a change in the deposition of the secondary cell wall thickenings (SWT) in the endothecium cells, from the normal longitudinal orientation to a transverse orientation, and hindered septum disintegration. These changes occurred only at the high temperature regime. The reorientation of SWT in GRC was accompanied by a similar change in microtubule orientation. A similar reorientation of microtubules was also observed in Arabidopsis (Arabidopsis thaliana) seedlings expressing green fluorescent protein tubulin (tubulin α 6) following glyphosate treatment. Glyphosate treatment induced the accumulation of high levels of indole-3-acetic acid in GRC anthers. Cotton plants treated with 2,4-dichlorophenoxyacetic acid had male sterile flowers, with SWT abnormalities in the endothecium layer similar to those observed in glyphosate-treated plants. Our data demonstrate that glyphosate inhibits anther dehiscence by inducing changes in the microtubule and cell wall organization in the endothecium cells, which are mediated by auxin. © 2006 American Society of Plant Biologists.
Note:
Related Files :
arabidopsis
Genetics
Gossypium
pollen (external)
temperature
tissue culture
Show More
Related Content
More details
DOI :
10.1104/pp.106.081943
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31439
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
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Scientific Publication
Glyphosate-induced anther indehiscence in cotton is partially temperature dependent and involves cytoskeleton and secondary wall modifications and auxin accumulation
141
Yasuor, H., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Abu-Abied, M., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Madmony, A., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sadot, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Riov, J., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Rubin, B., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Glyphosate-induced anther indehiscence in cotton is partially temperature dependent and involves cytoskeleton and secondary wall modifications and auxin accumulation
Yield reduction caused by late application of glyphosate to glyphosate-resistant cotton (Gossypium hirsutum; GRC) expressing CP4 5-enol-pyruvylshikmate-3-P synthase under the cauliflower mosaic virus-35S promoter has been attributed to male sterility. This study was aimed to elucidate the factors and mechanisms involved in this phenomenon. Western and tissue-print blots demonstrated a reduced expression of the transgene in anthers of GRC compared to ovules of the same plants. Glyphosate application to GRC grown at a high temperature regime after the initiation of flower buds caused a complete loss of pollen viability and inhibition of anther dehiscence, while at a moderate temperature regime only 50% of the pollen grains were disrupted and anther dehiscence was normal. Glyphosate-damaged anthers exhibited a change in the deposition of the secondary cell wall thickenings (SWT) in the endothecium cells, from the normal longitudinal orientation to a transverse orientation, and hindered septum disintegration. These changes occurred only at the high temperature regime. The reorientation of SWT in GRC was accompanied by a similar change in microtubule orientation. A similar reorientation of microtubules was also observed in Arabidopsis (Arabidopsis thaliana) seedlings expressing green fluorescent protein tubulin (tubulin α 6) following glyphosate treatment. Glyphosate treatment induced the accumulation of high levels of indole-3-acetic acid in GRC anthers. Cotton plants treated with 2,4-dichlorophenoxyacetic acid had male sterile flowers, with SWT abnormalities in the endothecium layer similar to those observed in glyphosate-treated plants. Our data demonstrate that glyphosate inhibits anther dehiscence by inducing changes in the microtubule and cell wall organization in the endothecium cells, which are mediated by auxin. © 2006 American Society of Plant Biologists.
Scientific Publication
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