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A pathogenicity gene isolated from the pPATH plasmid of Erwinia herbicola pv. gypsophilae Determines host specificity
Year:
1998
Authors :
Ezra, David
;
.
Volume :
11
Co-Authors:
Valinsky, L., Department of Plant Pathology, ARO, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel, Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis, S., Department of Plant Pathology, ARO, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Nizan, R., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Ezra, D., Department of Plant Pathology, ARO, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel, Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Facilitators :
From page:
753
To page:
762
(
Total pages:
10
)
Abstract:
The host range of the gall-forming bacterium Erwinia herbicola pv. gypsophilae (Ehg) is restricted to the gypsophila plant whereas E. herbicola pv. betae (Ehb) incites galls on beet as well as gypsophila. The pathogenicity of Ehg and Ehb was previously shown to be dependent on a plasmid (pPATH). Transposon mutagenesis was used to generate mutants on the cosmid pLA150 of the pPATH from Ehg824-1. A cluster of nonpathogenic mutations flanked by two IS1327 elements was identified on a 3.2-kb NdeI DNA fragment. All mutants were restored to pathogenicity by complementation in trans with the wild-type Ehg DNA. DNA sequence analysis of the 3.2-kb NdeI fragment revealed a single open reading frame (ORF) of 2 kb as well as a potential ribosome binding site and a putative hrp box upstream to the ORF. The ORF had no significant homology to known genes. Southern analysis also revealed the presence of DNA sequences that hybridized to the ORF in the beet pathovar Ehb4188. This gene was isolated and sequenced. Marker exchange mutants generated in the ORF of Ehb eliminated the pathogenicity of Ehb on gypsophila but fully retained its pathogenicity on beet. Since the putative gene appeared to encode a host-specific virulence factor for gypsophila it was designated as hsvG.
Note:
Related Files :
Base Sequence
Erwinia
mutation
Plant Diseases
sequence analysis
Sequence Homology, Amino Acid
virulence
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31463
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
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Scientific Publication
A pathogenicity gene isolated from the pPATH plasmid of Erwinia herbicola pv. gypsophilae Determines host specificity
11
Valinsky, L., Department of Plant Pathology, ARO, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel, Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis, S., Department of Plant Pathology, ARO, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Nizan, R., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Ezra, D., Department of Plant Pathology, ARO, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel, Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
A pathogenicity gene isolated from the pPATH plasmid of Erwinia herbicola pv. gypsophilae Determines host specificity
The host range of the gall-forming bacterium Erwinia herbicola pv. gypsophilae (Ehg) is restricted to the gypsophila plant whereas E. herbicola pv. betae (Ehb) incites galls on beet as well as gypsophila. The pathogenicity of Ehg and Ehb was previously shown to be dependent on a plasmid (pPATH). Transposon mutagenesis was used to generate mutants on the cosmid pLA150 of the pPATH from Ehg824-1. A cluster of nonpathogenic mutations flanked by two IS1327 elements was identified on a 3.2-kb NdeI DNA fragment. All mutants were restored to pathogenicity by complementation in trans with the wild-type Ehg DNA. DNA sequence analysis of the 3.2-kb NdeI fragment revealed a single open reading frame (ORF) of 2 kb as well as a potential ribosome binding site and a putative hrp box upstream to the ORF. The ORF had no significant homology to known genes. Southern analysis also revealed the presence of DNA sequences that hybridized to the ORF in the beet pathovar Ehb4188. This gene was isolated and sequenced. Marker exchange mutants generated in the ORF of Ehb eliminated the pathogenicity of Ehb on gypsophila but fully retained its pathogenicity on beet. Since the putative gene appeared to encode a host-specific virulence factor for gypsophila it was designated as hsvG.
Scientific Publication
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