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Transformation of carnation by microprojectile bombardment
Year:
1995
Source of publication :
Scientia Horticulturae
Authors :
Watad, Abed A.
;
.
Volume :
64
Co-Authors:
Zuker, A., The Kennedy-Leigh Center for Horticultural Research and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Chang, P.-F.L., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Ahroni, A., The Kennedy-Leigh Center for Horticultural Research and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Cheah, K., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Woodson, W.R., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Bressan, R.A., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Watad, A.A., Department of Ornamental Horticulture, The Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Hasegawa, P.M., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Vainstein, A., The Kennedy-Leigh Center for Horticultural Research and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Facilitators :
From page:
177
To page:
185
(
Total pages:
9
)
Abstract:
Transgenic carnation (Dianthus caryophyllus L.) plants were produced by microprojectile bombardment of highly regenerative stem segments. A two-step regeneration procedure based on the use of two different cytokinins-6-benzylaminopurine and thidiazuron-was employed for the production of adventitious shoots from stem segments. The size of the original stem was found to affect the regeneration efficiency of stem segments: the highest efficiency of adventitious shoot regeneration was obtained with segments originating from stems with two mature leaves, as compared to those with four, six or eight mature leaves. The tissue culture procedure was shown to be suitable for a number of standard and spray cultivars. Bombardment of cultivar "White Sim" stem segments was performed with a plasmid containing uidA and bar genes encoding β-glucuronidase and phosphinothricin-acetyltransferase, respectively. Transformation frequency was determined, based on the transient expression of uidA in stem segments. Following selection in the presence of the herbicide bialaphos, about 70 plantlets per 100 stem segments were recovered. Upon analysis, about 3% of these recovered plantlets exhibited strong stable uidA expression throughout the plant. Presence of the bar gene in plants stably expressing uidA was confirmed by Southern blot analysis. © 1995.
Note:
Related Files :
Adventitious shoot regeneration
Bialaphos
carnation
Dianthus caryophyllus
microprojectile bombardment
Stable transformation
Show More
Related Content
More details
DOI :
10.1016/0304-4238(95)00817-9
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31622
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:04
Scientific Publication
Transformation of carnation by microprojectile bombardment
64
Zuker, A., The Kennedy-Leigh Center for Horticultural Research and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Chang, P.-F.L., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Ahroni, A., The Kennedy-Leigh Center for Horticultural Research and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Cheah, K., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Woodson, W.R., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Bressan, R.A., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Watad, A.A., Department of Ornamental Horticulture, The Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Hasegawa, P.M., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Vainstein, A., The Kennedy-Leigh Center for Horticultural Research and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Transformation of carnation by microprojectile bombardment
Transgenic carnation (Dianthus caryophyllus L.) plants were produced by microprojectile bombardment of highly regenerative stem segments. A two-step regeneration procedure based on the use of two different cytokinins-6-benzylaminopurine and thidiazuron-was employed for the production of adventitious shoots from stem segments. The size of the original stem was found to affect the regeneration efficiency of stem segments: the highest efficiency of adventitious shoot regeneration was obtained with segments originating from stems with two mature leaves, as compared to those with four, six or eight mature leaves. The tissue culture procedure was shown to be suitable for a number of standard and spray cultivars. Bombardment of cultivar "White Sim" stem segments was performed with a plasmid containing uidA and bar genes encoding β-glucuronidase and phosphinothricin-acetyltransferase, respectively. Transformation frequency was determined, based on the transient expression of uidA in stem segments. Following selection in the presence of the herbicide bialaphos, about 70 plantlets per 100 stem segments were recovered. Upon analysis, about 3% of these recovered plantlets exhibited strong stable uidA expression throughout the plant. Presence of the bar gene in plants stably expressing uidA was confirmed by Southern blot analysis. © 1995.
Scientific Publication
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