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Journal of Heredity
Granevitze, Z., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Blum, S., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Cheng, H., USDA-ARS, Avian Disease and Oncology Laboratory, 3606 E. Mount Hope Road East, Lansing, MI 48823, United States
Vignal, A., Laboratoire de Genetique Cellulaire, INRA, Castanet-Tolosan 31326, France
Morisson, M., Laboratoire de Genetique Cellulaire, INRA, Castanet-Tolosan 31326, France
Ben-Ari, G., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
David, L., Department of Animal Sciences, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Feldman, M.W., Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, United States
Weigend, S., Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Neustadt 31535, Germany
Hillel, J., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks. © The American Genetic Association. 2007. All rights reserved.
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Female-specific DNA sequences in the chicken genome
98
Granevitze, Z., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Blum, S., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Cheng, H., USDA-ARS, Avian Disease and Oncology Laboratory, 3606 E. Mount Hope Road East, Lansing, MI 48823, United States
Vignal, A., Laboratoire de Genetique Cellulaire, INRA, Castanet-Tolosan 31326, France
Morisson, M., Laboratoire de Genetique Cellulaire, INRA, Castanet-Tolosan 31326, France
Ben-Ari, G., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
David, L., Department of Animal Sciences, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Feldman, M.W., Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, United States
Weigend, S., Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Neustadt 31535, Germany
Hillel, J., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Female-specific DNA sequences in the chicken genome
Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks. © The American Genetic Association. 2007. All rights reserved.
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