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The presence and formation of 5-dihydrotestosterone in rat testes in vivo and in vitro
Year:
1972
Source of publication :
Endocrinology
Authors :
Folman, Yeshayahu
;
.
Volume :
91
Co-Authors:
Folman, Y., Division of Physiology and Biochemistry of Reproduction, Department of Physiology, University of Southern California, School of Medicine, NewLos Angelesington, CA, 90033, United States, Volcani Institute of Agricultural Research, Bet-Dagan, Israel
Sowell, J.G., Division of Physiology and Biochemistry of Reproduction, Department of Physiology, University of Southern California, School of Medicine, NewLos Angelesington, CA, 90033, United States
Eik-Nes, K.B., Division of Physiology and Biochemistry of Reproduction, Department of Physiology, University of Southern California, School of Medicine, NewLos Angelesington, CA, 90033, United States
Facilitators :
From page:
702
To page:
710
(
Total pages:
9
)
Abstract:
5α-Dihydrotestosterone (DHT) was extracted, separated and identified from testes of mature rats. When teased preparations of testicular tissue, levator ani or thigh muscle were incubated with 3H-testosterone (T) and an NADPH generating system, 3H-DHT could be detected. Teased testicular tissue produced more 3H-DHT from 3H-T than homogenized tissue and the rates of this conversion in teased testicular tissue were the same in incubations carried out at 33 C or at 37 C. Testes of immature rats formed larger quantities of 3H-DHT and other 5a-reduced metabolites of 3H-T than testes of mature animals. FSH tended to stimulate testicular production of 3H-DHT from 3H-T only when administered to the rats prior to removal of the testes. Testes from rats injected with hCG formed less 3H-DHT from 3H-T than testes from control animals. This effect was also observed when hCG was added to the incubation medium. LH (ICSH) did not have an effect on synthesis of DHT from T at the doses tested. When testicular tissue was incubated with 3H-DHT as the substrate, the main 3H-metabolite was androstanediol. © 1972 by The Endocrine Society.
Note:
Related Files :
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biosynthesis
Follicle Stimulating Hormone
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metabolism
temperature
testis
tritium
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More details
DOI :
10.1210/endo-91-3-702
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
32040
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
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Scientific Publication
The presence and formation of 5-dihydrotestosterone in rat testes in vivo and in vitro
91
Folman, Y., Division of Physiology and Biochemistry of Reproduction, Department of Physiology, University of Southern California, School of Medicine, NewLos Angelesington, CA, 90033, United States, Volcani Institute of Agricultural Research, Bet-Dagan, Israel
Sowell, J.G., Division of Physiology and Biochemistry of Reproduction, Department of Physiology, University of Southern California, School of Medicine, NewLos Angelesington, CA, 90033, United States
Eik-Nes, K.B., Division of Physiology and Biochemistry of Reproduction, Department of Physiology, University of Southern California, School of Medicine, NewLos Angelesington, CA, 90033, United States
The presence and formation of 5-dihydrotestosterone in rat testes in vivo and in vitro
5α-Dihydrotestosterone (DHT) was extracted, separated and identified from testes of mature rats. When teased preparations of testicular tissue, levator ani or thigh muscle were incubated with 3H-testosterone (T) and an NADPH generating system, 3H-DHT could be detected. Teased testicular tissue produced more 3H-DHT from 3H-T than homogenized tissue and the rates of this conversion in teased testicular tissue were the same in incubations carried out at 33 C or at 37 C. Testes of immature rats formed larger quantities of 3H-DHT and other 5a-reduced metabolites of 3H-T than testes of mature animals. FSH tended to stimulate testicular production of 3H-DHT from 3H-T only when administered to the rats prior to removal of the testes. Testes from rats injected with hCG formed less 3H-DHT from 3H-T than testes from control animals. This effect was also observed when hCG was added to the incubation medium. LH (ICSH) did not have an effect on synthesis of DHT from T at the doses tested. When testicular tissue was incubated with 3H-DHT as the substrate, the main 3H-metabolite was androstanediol. © 1972 by The Endocrine Society.
Scientific Publication
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