אסיף מאגר המחקר החקלאי

Multiple species of defective RNAs in plants infected with citrus tristeza virus

Year:

1995

Source of publication :

Authors :

Volume :

214

Co-Authors:

Mawass, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Mietkiewska, E., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Hilf, M.E., Horticultural Research Laboratory, USDA-ARS, 2120 Camden Road, Orlando, FL 33803, United States
Ashoulin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Karasev, A.V., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Gafny, R., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Lee, R.F., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Garnsey, S.M., Horticultural Research Laboratory, USDA-ARS, 2120 Camden Road, Orlando, FL 33803, United States
Dawson, W.O., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Bar-Joseph, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel

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Abstract:

Alemow (Citrus macrophylla) and sweet orange (C. sinensis) plants infected, respectively, with several lsraeli and Florida isolates of the citrus tristeza virus (CTV) were found to contain multiple species of RNA molecules with features similar to defective-interfering RNAs. Northern blot hybridizations of dsRNAs extracted from serial passages of the Israeli VT isolate (CTV-VT) and from different plants infected with a single source of inoculum showed considerable variation both in the presence and in the relative abundance of the defective RNA (D-RNA) bands. The D-RNA molecules were found to be encapsidated in the CTV particles. Sequence analysis of two VT D-RNA molecules of 2.7 and 4.5 kb revealed that they were composed of two regions corresponding to 1818 and 4036 nucleotides from the 5' and 938 and 442 nucleotides from the 3' termini of the CTV-VT genomic RNA, respectively. A short (ca. 0.8 kb) nonencapsidated single-stranded positive-sense RNA species was also found in infected plants. This ssRNA, which copurified with dsRNAs, was shown by hybridization to encompass the 5'-terminal part of the CTV genome and might have an extensive secondary structure.

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