נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
An orange ripening mutant links plastid NAD(P)H dehydrogenase complex activity to central and specialized metabolism during tomato fruit maturation
Year:
2010
Source of publication :
Plant Cell
Authors :
Itkin, Maxim
;
.
Volume :
22
Co-Authors:
Nashilevitz, S., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Faculty of Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Melamed-Bessudo, C., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Izkovich, Y., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Rogachev, I., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Osorio, S., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Itkin, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Adato, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Pankratov, I., Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Hirschberg, J., Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Fernie, A.R., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Wolf, S., Faculty of Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Usadel, B., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Levy, A.A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Rumeau, D., Commissariat à l'Energie Atomique Cadarache, Direction des Sciences du Vivant, Institut de Biologie Environnementale et Biotechnologie, Centre National de la Recherche Scientifique/Commissariat à l'Energie Atomique, Unité Mixte de Recherche 6191, F-13108 Saint-Paul-lez-Durance, France
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
1977
To page:
1997
(
Total pages:
21
)
Abstract:
In higher plants, the plastidial NADH dehydrogenase (Ndh) complex supports nonphotochemical electron fluxes from stromal electron donors to plastoquinones. Ndh functions in chloroplasts are not clearly established; however, its activity was linked to the prevention of the overreduction of stroma, especially under stress conditions. Here, we show by the characterization of OrrDs, a dominant transposon-tagged tomato (Solanum lycopersicum) mutant deficient in the NDH-M subunit, that this complex is also essential for the fruit ripening process. Alteration to the NDH complex in fruit changed the climacteric, ripening-associated metabolites and transcripts as well as fruit shelf life. Metabolic processes in chromoplasts of ripening tomato fruit were affected in OrrDs, as mutant fruit were yellow-orange and accumulated substantially less total carotenoids, mainly β-carotene and lutein. The changes in carotenoids were largely influenced by environmental conditions and accompanied by modifications in levels of other fruit antioxidants, namely, flavonoids and tocopherols. In contrast with the pigmentation phenotype in mature mutant fruit, OrrDs leaves and green fruits did not display a visible phenotype but exhibited reduced Ndh complex quantity and activity. This study therefore paves the way for further studies on the role of electron transport and redox reactions in the regulation of fruit ripening and its associated metabolism © 2010 American Society of Plant Biologists.
Note:
Related Files :
carotenoids
flavonoids
Genetics
metabolism
mutation
phenotype
reduced nicotinamide adenine dinucleotide dehydrogenase
Show More
Related Content
More details
DOI :
10.1105/tpc.110.074716
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
32572
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
Scientific Publication
An orange ripening mutant links plastid NAD(P)H dehydrogenase complex activity to central and specialized metabolism during tomato fruit maturation
22
Nashilevitz, S., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Faculty of Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Melamed-Bessudo, C., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Izkovich, Y., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Rogachev, I., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Osorio, S., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Itkin, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Adato, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Pankratov, I., Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Hirschberg, J., Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Fernie, A.R., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Wolf, S., Faculty of Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Usadel, B., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
Levy, A.A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Rumeau, D., Commissariat à l'Energie Atomique Cadarache, Direction des Sciences du Vivant, Institut de Biologie Environnementale et Biotechnologie, Centre National de la Recherche Scientifique/Commissariat à l'Energie Atomique, Unité Mixte de Recherche 6191, F-13108 Saint-Paul-lez-Durance, France
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
An orange ripening mutant links plastid NAD(P)H dehydrogenase complex activity to central and specialized metabolism during tomato fruit maturation
In higher plants, the plastidial NADH dehydrogenase (Ndh) complex supports nonphotochemical electron fluxes from stromal electron donors to plastoquinones. Ndh functions in chloroplasts are not clearly established; however, its activity was linked to the prevention of the overreduction of stroma, especially under stress conditions. Here, we show by the characterization of OrrDs, a dominant transposon-tagged tomato (Solanum lycopersicum) mutant deficient in the NDH-M subunit, that this complex is also essential for the fruit ripening process. Alteration to the NDH complex in fruit changed the climacteric, ripening-associated metabolites and transcripts as well as fruit shelf life. Metabolic processes in chromoplasts of ripening tomato fruit were affected in OrrDs, as mutant fruit were yellow-orange and accumulated substantially less total carotenoids, mainly β-carotene and lutein. The changes in carotenoids were largely influenced by environmental conditions and accompanied by modifications in levels of other fruit antioxidants, namely, flavonoids and tocopherols. In contrast with the pigmentation phenotype in mature mutant fruit, OrrDs leaves and green fruits did not display a visible phenotype but exhibited reduced Ndh complex quantity and activity. This study therefore paves the way for further studies on the role of electron transport and redox reactions in the regulation of fruit ripening and its associated metabolism © 2010 American Society of Plant Biologists.
Scientific Publication
You may also be interested in