נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Protoplast culture: Viruses and viroids
Year:
2017
Authors :
Gera, Abdullah
;
.
Volume :
Co-Authors:
Facilitators :
From page:
313
To page:
327
(
Total pages:
15
)
Abstract:

Isolated protoplasts have been described as “naked” plant cells because the cell wall has been experimentally removed by either a mechanical or an enzymatic digestion.1 They provide a unique system for clarifying the interactions between a plant virus and its host at the cellular level. It was in 1960 that a cell wall-degrading enzyme was successfully used by Cocking2 to isolate protoplasts from tomato. The crucial advance in the field came in 1968 when Takebe et al.3 were able to solve the fundamental difficulties in preparing protoplasts from leaf mesophyll and gave strong evidence for substantial tobacco mosaic virus (TMV) replication resulting from infection with TMV 3 - 5 and TMV-RNA.6 Subsequent to 314infection by TMV, a rod-shaped virus, mesophyll protoplasts have been successfully infected with spherical viruses, such as cucumber mosaic virus (CMV), 7 filamentous viruses such as potato virus X (PVX), 8 multicomponent viruses such as brome mosaic virus (BMV), 9, 10 and rhadoviruses such as sonchus yellow net virus (SYNV). 11 © 1995 by CRC Press, Inc.

Note:
Related Files :
Plant Pathology
protoplast culture
viruses and viroids
Show More
Related Content
More details
DOI :
10.1201/9780203746523
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
Book chapter
;
.
Language:
English
Editors' remarks:
ID:
37025
Last updated date:
02/03/2022 17:27
Creation date:
28/08/2018 12:20
You may also be interested in
Scientific Publication
Protoplast culture: Viruses and viroids
Protoplast culture: Viruses and viroids .

Isolated protoplasts have been described as “naked” plant cells because the cell wall has been experimentally removed by either a mechanical or an enzymatic digestion.1 They provide a unique system for clarifying the interactions between a plant virus and its host at the cellular level. It was in 1960 that a cell wall-degrading enzyme was successfully used by Cocking2 to isolate protoplasts from tomato. The crucial advance in the field came in 1968 when Takebe et al.3 were able to solve the fundamental difficulties in preparing protoplasts from leaf mesophyll and gave strong evidence for substantial tobacco mosaic virus (TMV) replication resulting from infection with TMV 3 - 5 and TMV-RNA.6 Subsequent to 314infection by TMV, a rod-shaped virus, mesophyll protoplasts have been successfully infected with spherical viruses, such as cucumber mosaic virus (CMV), 7 filamentous viruses such as potato virus X (PVX), 8 multicomponent viruses such as brome mosaic virus (BMV), 9, 10 and rhadoviruses such as sonchus yellow net virus (SYNV). 11 © 1995 by CRC Press, Inc.

Scientific Publication
You may also be interested in