Powdery mildew, caused by Podosphaera aphanis, is one of the major fungal diseases of cultivated strawberry (Fragaria× ananassa Duchense) world-wide. In the present research we report the efficacy of various control agents in induced resistance in strawberry plants against Powdery mildew. Inducers tested are Trichoderma harzianum T39, acibenzolar-S-methyl (Bion), a new protein hydrolysate inducer, an isolate of a bacterium and one of a yeast, and biochar. These agents induced systemic resistance when applied to the root zone, while the disease was found and measured on the leaves. In addition, we studied the effect of T39, Bion and biochar on plant defense related gene expression. We show that while Bion induced Systemic Acquired Resistance (SAR) only, as expected, T39 as well as biochar induced SAR and Induced Systemic Resistance (ISR).