Advanced Search
IOBC/WPRS Bulletin

S Shafir, M Abu-Toamy

The present study involved the biocontrol preparation TRICHODEX (Trichoderma
harzianum T39) and for treating flowers of strawberry plants against the fungal pathogen Botrytis
cinerea infection and development of flower and fruit grey mould. Biocontrol of strawberry fruit grey
mould by spraying the biocontrol preparation is ineffective unless very frequent applications are done
or high concentrations are used. Initially we evaluated the relative efficiency of four powder
dispensers in loading honeybees with the T. harzianum T39 preparation. The newly developed
‘Triwaks’ dispenser was found more effective than three other dispenser types, ‘Peng’, ‘Tub’ and
‘Harwood’, with respect to effects of the dispensers on bee activity and effectiveness loading bees
with high levels of Trichoderma inoculum for a long period of time after its application. Bees leaving
hives equipped with the Triwaks dispenser that was better than the others, were loaded at the most
with 1.45*105 cfu T. harzianum T39/bee. Strawberry flowers in a commercial polyethylene
greenhouse located 24 meter far from hives with Trichoderma loaded dispenser had 3.6*103
cfu/flower. Similar level of Trichoderma was found on flowers immediately after Trichoderma spray,
but the level declined with time. These levels were found necessary for the control of strawberry grey
mould. Ten beehives were placed at the edge of a commercial strawberry field and the biocontrol
preparation Trichodex was added to their dispensers on a daily basis from mid December until mid
March. Experimental plots were located 25-50 m from the hives. During the period of grey mould
development, bee delivered T. harzianum T39 was found better than season long chemical treatment
(fludioxonil+cyprodinil and pyrimethanil). It can be concluded that the delivery of Trichoderma by
bees to strawberry flowers is effective in strawberry fruit grey mould control and can be integrated in
an IPM scheme in this crop.

Powered by ClearMash Solutions Ltd -
Volcani treasures
About
Terms of use
Using honeybees to deliver a biocontrol agent for the control of strawberry Botrytis cinerea-fruit rots
27 (8)

S Shafir, M Abu-Toamy

The present study involved the biocontrol preparation TRICHODEX (Trichoderma
harzianum T39) and for treating flowers of strawberry plants against the fungal pathogen Botrytis
cinerea infection and development of flower and fruit grey mould. Biocontrol of strawberry fruit grey
mould by spraying the biocontrol preparation is ineffective unless very frequent applications are done
or high concentrations are used. Initially we evaluated the relative efficiency of four powder
dispensers in loading honeybees with the T. harzianum T39 preparation. The newly developed
‘Triwaks’ dispenser was found more effective than three other dispenser types, ‘Peng’, ‘Tub’ and
‘Harwood’, with respect to effects of the dispensers on bee activity and effectiveness loading bees
with high levels of Trichoderma inoculum for a long period of time after its application. Bees leaving
hives equipped with the Triwaks dispenser that was better than the others, were loaded at the most
with 1.45*105 cfu T. harzianum T39/bee. Strawberry flowers in a commercial polyethylene
greenhouse located 24 meter far from hives with Trichoderma loaded dispenser had 3.6*103
cfu/flower. Similar level of Trichoderma was found on flowers immediately after Trichoderma spray,
but the level declined with time. These levels were found necessary for the control of strawberry grey
mould. Ten beehives were placed at the edge of a commercial strawberry field and the biocontrol
preparation Trichodex was added to their dispensers on a daily basis from mid December until mid
March. Experimental plots were located 25-50 m from the hives. During the period of grey mould
development, bee delivered T. harzianum T39 was found better than season long chemical treatment
(fludioxonil+cyprodinil and pyrimethanil). It can be concluded that the delivery of Trichoderma by
bees to strawberry flowers is effective in strawberry fruit grey mould control and can be integrated in
an IPM scheme in this crop.

Scientific Publication
You may also be interested in