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אסיף מאגר המחקר החקלאי
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Development of Immunochemical Methods for Purification and Detection of the Steroid Drug Medroxyprogesterone Acetate
Year:
2012
Authors :
Altstein, Miriam
;
.
Bronshtein, Alisa
;
.
Volume :
3 (7)
Co-Authors:

Alex Krol, Haim Schlesinger

Facilitators :
From page:
624
To page:
639
(
Total pages:
16
)
Abstract:

An immunochemical sol-gel-based immunoaffinity purification (IAP) method for purification and detection of the progestin drug medroxyprogesterone acetate (MPA) was developed. A polyclonal antibody (Ab) for MPA was generated, and two competitive (indirect and direct) sensitive enzyme-linked immunosorbent assays (ELISAs) for its detection were developed and implemented to determine the recovery and efficiency of the sol-gel based IAP method. The detection limits of the assays were 1.4 ± 0.2 ng∙mL−1 (n = 4) and 4.0 ± 0.4 ng∙mL−1 (n = 25) for the indirect and direct ELISAs, respectively. The Abs did not exhibit cross-reactivity with any other progestin or steroid hormone, with the exception of megestrol acetate, with which the Ab exhibited 76% cross-reactivity. The sol-gel IAP method successfully eliminated serum interference to a degree that enabled ELISA analysis of spiked serum samples. This method was also found fully compatible with subsequent chemical analytical methods, such as liquid chromatography followed by mass spectrometry (LC-MS/MS). The approaches developed in this study form a basis for analysis of MPA in biological samples and may be further used to study population exposure to MPA and to monitor MPA contamination in water samples.

Note:
Related Files :
ELISA
Immunoaffinity chromatography
Medroxyprogesterone acetate
Pharmaceutical residues
Residue monitoring
Sol-gel
Show More
Related Content
More details
DOI :
DOI:10.4236/jep.2012.37076
Article number:
0
Affiliations:
Database:
Google Scholar
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
37722
Last updated date:
02/03/2022 17:27
Creation date:
28/10/2018 12:19
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Scientific Publication
Development of Immunochemical Methods for Purification and Detection of the Steroid Drug Medroxyprogesterone Acetate
3 (7)

Alex Krol, Haim Schlesinger

Development of Immunochemical Methods for Purification and Detection of the Steroid Drug Medroxyprogesterone Acetate

An immunochemical sol-gel-based immunoaffinity purification (IAP) method for purification and detection of the progestin drug medroxyprogesterone acetate (MPA) was developed. A polyclonal antibody (Ab) for MPA was generated, and two competitive (indirect and direct) sensitive enzyme-linked immunosorbent assays (ELISAs) for its detection were developed and implemented to determine the recovery and efficiency of the sol-gel based IAP method. The detection limits of the assays were 1.4 ± 0.2 ng∙mL−1 (n = 4) and 4.0 ± 0.4 ng∙mL−1 (n = 25) for the indirect and direct ELISAs, respectively. The Abs did not exhibit cross-reactivity with any other progestin or steroid hormone, with the exception of megestrol acetate, with which the Ab exhibited 76% cross-reactivity. The sol-gel IAP method successfully eliminated serum interference to a degree that enabled ELISA analysis of spiked serum samples. This method was also found fully compatible with subsequent chemical analytical methods, such as liquid chromatography followed by mass spectrometry (LC-MS/MS). The approaches developed in this study form a basis for analysis of MPA in biological samples and may be further used to study population exposure to MPA and to monitor MPA contamination in water samples.

Scientific Publication
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