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Diversity of Nitrate-Reducing and Denitrifying Bacteria in a Marine Aquaculture Biofilter and their Response to Sulfide
Year:
2006
Authors :
Cytryn, Eddie
;
.
Minz, Dror
;
.
Volume :
Co-Authors:

Krieger, Bärbel; Schwermer, Carsten U.; Rezakhani, Nastaran; Horn, Marcus A.; Gieseke, Armin; van Rijn, Jaap; Drake, Harold L.; Schramm, Andreas

Facilitators :
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0
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Total pages:
1
)
Abstract:

Conventional aquaculture systems release nitrogen compounds and organic matter into marine environments. As an environmentally-friendly alternative, a zero-discharge mariculture system recently was developed containing a 3-stage biofilter for nitrification, denitrification/anaerobic sludge digestion, and sulfide oxidation. Sulfate reduction in the anaerobic part of the system leads to sulfide concentrations exceeding 5 mM, which may affect nitrate reduction and denitrification. Sulfide can inhibit nitrous oxide reductase, trigger a shift from denitrification to dissimilatory nitrate reduction to ammonium (DNRA), or be used as electron donor for nitrate reduction. The goal of this study was to identify and isolate nitrate-reducing and denitrifying bacteria from the biofilter and to investigate their response to sulfide concentrations relevant for the system. Almost 500 nitrate-consuming isolates were screened by 16S rRNA gene-RFLP; for each RFLP pattern representatives were sequenced. In total, 40 different strains were identified, some of them novel species, mostly affiliating with Alphaproteobacteria but also including Beta- and Gammaproteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The diversity of the isolates was compared to the cultivation-independent diversity of nitrate-reducing and denitrifying bacteria based on narG and nosZ as functional marker genes. Growth experiments revealed great differences in sulfide-tolerance among isolates, ranging from < 50 µM to 5 mM; some strains were also able to oxidize sulfide. Increasing sulfide concentrations generally resulted in increased nitrous oxide production. Batch incubations of anaerobic sludge with 15N-nitrate confirmed the in situ relevance of these results and indicated a sulfide-induced shift from denitrification to DNRA.

Note:
Related Files :
aquaculture
bacteria
Biofilters
DENITRIFICATION
Marine aquaculture
nitrate
sulfide
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Article number:
0
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Database:
Google Scholar
Publication Type:
Conference paper
;
.
article
;
.
Language:
English
Editors' remarks:
ID:
41405
Last updated date:
02/03/2022 17:27
Creation date:
18/06/2019 10:59
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Scientific Publication
Diversity of Nitrate-Reducing and Denitrifying Bacteria in a Marine Aquaculture Biofilter and their Response to Sulfide

Krieger, Bärbel; Schwermer, Carsten U.; Rezakhani, Nastaran; Horn, Marcus A.; Gieseke, Armin; van Rijn, Jaap; Drake, Harold L.; Schramm, Andreas

Diversity of Nitrate-Reducing and Denitrifying Bacteria in a Marine Aquaculture Biofilter and their Response to Sulfide

Conventional aquaculture systems release nitrogen compounds and organic matter into marine environments. As an environmentally-friendly alternative, a zero-discharge mariculture system recently was developed containing a 3-stage biofilter for nitrification, denitrification/anaerobic sludge digestion, and sulfide oxidation. Sulfate reduction in the anaerobic part of the system leads to sulfide concentrations exceeding 5 mM, which may affect nitrate reduction and denitrification. Sulfide can inhibit nitrous oxide reductase, trigger a shift from denitrification to dissimilatory nitrate reduction to ammonium (DNRA), or be used as electron donor for nitrate reduction. The goal of this study was to identify and isolate nitrate-reducing and denitrifying bacteria from the biofilter and to investigate their response to sulfide concentrations relevant for the system. Almost 500 nitrate-consuming isolates were screened by 16S rRNA gene-RFLP; for each RFLP pattern representatives were sequenced. In total, 40 different strains were identified, some of them novel species, mostly affiliating with Alphaproteobacteria but also including Beta- and Gammaproteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The diversity of the isolates was compared to the cultivation-independent diversity of nitrate-reducing and denitrifying bacteria based on narG and nosZ as functional marker genes. Growth experiments revealed great differences in sulfide-tolerance among isolates, ranging from < 50 µM to 5 mM; some strains were also able to oxidize sulfide. Increasing sulfide concentrations generally resulted in increased nitrous oxide production. Batch incubations of anaerobic sludge with 15N-nitrate confirmed the in situ relevance of these results and indicated a sulfide-induced shift from denitrification to DNRA.

Scientific Publication
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