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Using fluorescent labeling for tracking Bacillus subtilis growth in milk
Year:
2019
Source of publication :
Volcani Voice
Authors :
Inbar, Dana
;
.
Shemesh, Moshe
;
.
Volume :
6
Co-Authors:
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Many types of bacteria proliferate in milk, as it provides an ideal growth medium due to its high nutritional value. The opaqueness of  milk does not enable measuring of optical density to  determine bacterial growth, which significantly lengthens analysis of bacterial growth in milk for food safety and basic research purposes. We developed a unique real-time fluorescence based  method for tracking bacterial growth in milk, which applies a microplate reader and direct microscopic observation. A fluorescently-tagged Bacillus subtilis was chosen as a model organism for this assay since this bacterium is ubiquitous in milk and its products. We suggest that the developed assay can be applied for rapid detection of B. subtilis growth in milk.        

Note:
Related Files :
bacillus subtilis
bacteria
Fluorescent
milk
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DOI :
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
41593
Last updated date:
02/03/2022 17:27
Creation date:
23/06/2019 11:55
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Using fluorescent labeling for tracking Bacillus subtilis growth in milk
6

Many types of bacteria proliferate in milk, as it provides an ideal growth medium due to its high nutritional value. The opaqueness of  milk does not enable measuring of optical density to  determine bacterial growth, which significantly lengthens analysis of bacterial growth in milk for food safety and basic research purposes. We developed a unique real-time fluorescence based  method for tracking bacterial growth in milk, which applies a microplate reader and direct microscopic observation. A fluorescently-tagged Bacillus subtilis was chosen as a model organism for this assay since this bacterium is ubiquitous in milk and its products. We suggest that the developed assay can be applied for rapid detection of B. subtilis growth in milk.        

Scientific Publication
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