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Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum
Year:
2019
Authors :
Aly, Radi
;
.
Droby, Samir
;
.
Feygenberg, Oleg
;
.
Levin, Elena
;
.
Rafael, Ginat
;
.
Volume :
7(6): 175
Co-Authors:

Jing Ma, John Norelli, Michael Wisniewski - Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Wiltshire Road, Kearneysville, WV 25430

Ana-Rosa Ballester, Luis Gonzalez-Candelas - Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Calle Catedrático Agustin Escardino 7, Paterna 46980, Valencia 46980, Spain

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Penicillium expansum is a major postharvest pathogen that infects different fruits, mainly through injuries inflicted during harvest or subsequent handling after harvest. Several effectors were suggested to mediate pathogenicity of P. expansum in fruit tissue. Among these effectors Nep1-like proteins (NLPs), produced by various microorganisms with different lifestyles, are known for their ability to induce necrosis in dicot plants and were shown to be involved in virulence of several plant-related pathogens. This study was aimed at the identification and functional characterization of two NLP genes found in the genome of P. expansum. The genes were designated Penlp1 and Penlp2 and were found to code type1 and type3 NLP respectively. Necrosis-inducing activity of the two proteins was demonstrated by transient expression in Nicotiana benthamiana leaves. While Penlp1 expression was induced during apple infection and in liquid culture, the highest level of Penlp2 expression was found in ungerminated spores. Deletion of Penlp1, but not Penlp2, resulted in reduced virulence on apples manifested by reduced rate of lesion development (disease severity).

Note:
Related Files :
Apple
Blue mold
Effectors
Nep1
Pathogenecity
Penicillium expansum
virulence
Show More
Related Content
More details
DOI :
10.3390/microorganisms7060175
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
42685
Last updated date:
02/03/2022 17:27
Creation date:
22/07/2019 09:36
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Scientific Publication
Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum
7(6): 175

Jing Ma, John Norelli, Michael Wisniewski - Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Wiltshire Road, Kearneysville, WV 25430

Ana-Rosa Ballester, Luis Gonzalez-Candelas - Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Calle Catedrático Agustin Escardino 7, Paterna 46980, Valencia 46980, Spain

Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum

Penicillium expansum is a major postharvest pathogen that infects different fruits, mainly through injuries inflicted during harvest or subsequent handling after harvest. Several effectors were suggested to mediate pathogenicity of P. expansum in fruit tissue. Among these effectors Nep1-like proteins (NLPs), produced by various microorganisms with different lifestyles, are known for their ability to induce necrosis in dicot plants and were shown to be involved in virulence of several plant-related pathogens. This study was aimed at the identification and functional characterization of two NLP genes found in the genome of P. expansum. The genes were designated Penlp1 and Penlp2 and were found to code type1 and type3 NLP respectively. Necrosis-inducing activity of the two proteins was demonstrated by transient expression in Nicotiana benthamiana leaves. While Penlp1 expression was induced during apple infection and in liquid culture, the highest level of Penlp2 expression was found in ungerminated spores. Deletion of Penlp1, but not Penlp2, resulted in reduced virulence on apples manifested by reduced rate of lesion development (disease severity).

Scientific Publication
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