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Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom
Year:
2019
Source of publication :
eLife
Authors :
Shapiro, Orr H.
;
.
Volume :
Co-Authors:

Avia Mizrachi, Shiri Graff van Creveld, Assaf Vardi - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel

Shilo Rosenwasser - 3The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Diatoms are photosynthetic microorganisms of great ecological and biogeochemical importance, forming vast blooms in aquatic ecosystems. However, we are still lacking fundamental understanding of how individual cells sense and respond to diverse stress conditions, and what acclimation strategies are employed during bloom dynamics. We investigated cellular responses to environmental stress at the single-cell level using the redox sensor roGFP targeted to various organelles in the diatom Phaeodactylum tricornutum. We detected cell-to-cell variability using flow cytometry cell sorting and a microfluidics system for live imaging of oxidation dynamics. Chloroplast-targeted roGFP exhibited a light-dependent, bi-stable oxidation pattern in response to H2O2 and high light, revealing distinct subpopulations of sensitive oxidized cells and resilient reduced cells. Early oxidation in the chloroplast preceded commitment to cell death, and can be used for sensing stress cues and regulating cell fate. We propose that light-dependent metabolic heterogeneity regulates diatoms’ sensitivity to environmental stressors in the ocean.

Note:
Related Files :
aquatic ecosystem
chloroplast
diatom
Redox
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Related Content
More details
DOI :
10.7554/eLife.47732
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
43181
Last updated date:
02/03/2022 17:27
Creation date:
12/08/2019 08:21
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Scientific Publication
Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom

Avia Mizrachi, Shiri Graff van Creveld, Assaf Vardi - Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel

Shilo Rosenwasser - 3The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel 

Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom

Diatoms are photosynthetic microorganisms of great ecological and biogeochemical importance, forming vast blooms in aquatic ecosystems. However, we are still lacking fundamental understanding of how individual cells sense and respond to diverse stress conditions, and what acclimation strategies are employed during bloom dynamics. We investigated cellular responses to environmental stress at the single-cell level using the redox sensor roGFP targeted to various organelles in the diatom Phaeodactylum tricornutum. We detected cell-to-cell variability using flow cytometry cell sorting and a microfluidics system for live imaging of oxidation dynamics. Chloroplast-targeted roGFP exhibited a light-dependent, bi-stable oxidation pattern in response to H2O2 and high light, revealing distinct subpopulations of sensitive oxidized cells and resilient reduced cells. Early oxidation in the chloroplast preceded commitment to cell death, and can be used for sensing stress cues and regulating cell fate. We propose that light-dependent metabolic heterogeneity regulates diatoms’ sensitivity to environmental stressors in the ocean.

Scientific Publication
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