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Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF Signaling, Suggesting that Their Interacting Adipokine Role is Unique to Mammals
Year:
2019
Authors :
Friedman-Einat, Miriam
;
.
Ganesan, Saibaba
;
.
Miyara, Shoval
;
.
Ruzal, Mark
;
.
Seroussi, Eyal
;
.
Yosefi, Sara
;
.
Volume :
Co-Authors:

Knytl, M., Department of Developmental Biology, Faculty of Science, Charles University, Prague, 12843, Czech Republic, Department of Biology, Faculty of Science, McMaster University, Hamilton, Canada; Pitel, F., GenPhySE, Université de Toulouse, INRA, ENVT, Castanet Tolosan, 31326, France; Elleder, D., Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, 14220, Czech Republic; Krylov, V., Department of Developmental Biology, Faculty of Science, Charles University, Prague, 12843, Czech Republic; Leroux, S., GenPhySE, Université de Toulouse, INRA, ENVT, Castanet Tolosan, 31326, France; Morisson, M., GenPhySE, Université de Toulouse, INRA, ENVT, Castanet Tolosan, 31326, France; Andersson, L., Department of Medical Biochemistry and Microbiology, Uppsala UniversityUppsala  SE-75123, Sweden, Department of Animal Breeding and Genetics, Swedish University of Agricultural SciencesUppsala  SE-75007, Sweden, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4458, USA;

Facilitators :
From page:
0
To page:
0
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Total pages:
1
)
Abstract:

In mammals, leptin and tumor-necrosis factor (TNF) are prominent interacting adipokines mediating appetite control and insulin sensitivity. While TNF pleiotropically functions in immune defense and cell survival, leptin is largely confined to signaling energy stores in adipocytes. Knowledge about the function of avian leptin and TNF is limited and they are absent or lowly expressed in adipose, respectively. Employing radiation-hybrid mapping and FISH-TSA, we mapped TNF and its syntenic genes to chicken chromosome 16 within the major histocompatibility complex (MHC) region. This mapping position suggests that avian TNF has a role in regulating immune response. To test its possible interaction with leptin within the immune system and beyond, we compared the transcription patterns of TNF, leptin and their cognate receptors obtained by meta-analysis of GenBank RNA-seq data. While expression of leptin and its receptor (LEPR) were detected in the brain and digestive tract, TNF and its receptor mRNAs were primarily found in viral-infected and LPS-treated leukocytes. We confirmed leptin expression in the duodenum by immunohistochemistry staining. Altogether, we suggest that whereas leptin and TNF interact as adipokines in mammals, in birds, they have distinct roles. Thus, the interaction between leptin and TNF may be unique to mammals.

Note:
Related Files :
chicken
Digestive Tract Contents
Duodenum
FISH-TSA
immune system
Leptin
radiation hybrid mapping
TNF
Show More
Related Content
More details
DOI :
10.3390/ijms20184489
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
44098
Last updated date:
02/03/2022 17:27
Creation date:
03/10/2019 14:37
Scientific Publication
Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF Signaling, Suggesting that Their Interacting Adipokine Role is Unique to Mammals

Knytl, M., Department of Developmental Biology, Faculty of Science, Charles University, Prague, 12843, Czech Republic, Department of Biology, Faculty of Science, McMaster University, Hamilton, Canada; Pitel, F., GenPhySE, Université de Toulouse, INRA, ENVT, Castanet Tolosan, 31326, France; Elleder, D., Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, 14220, Czech Republic; Krylov, V., Department of Developmental Biology, Faculty of Science, Charles University, Prague, 12843, Czech Republic; Leroux, S., GenPhySE, Université de Toulouse, INRA, ENVT, Castanet Tolosan, 31326, France; Morisson, M., GenPhySE, Université de Toulouse, INRA, ENVT, Castanet Tolosan, 31326, France; Andersson, L., Department of Medical Biochemistry and Microbiology, Uppsala UniversityUppsala  SE-75123, Sweden, Department of Animal Breeding and Genetics, Swedish University of Agricultural SciencesUppsala  SE-75007, Sweden, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4458, USA;

Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF Signaling, Suggesting that Their Interacting Adipokine Role is Unique to Mammals

In mammals, leptin and tumor-necrosis factor (TNF) are prominent interacting adipokines mediating appetite control and insulin sensitivity. While TNF pleiotropically functions in immune defense and cell survival, leptin is largely confined to signaling energy stores in adipocytes. Knowledge about the function of avian leptin and TNF is limited and they are absent or lowly expressed in adipose, respectively. Employing radiation-hybrid mapping and FISH-TSA, we mapped TNF and its syntenic genes to chicken chromosome 16 within the major histocompatibility complex (MHC) region. This mapping position suggests that avian TNF has a role in regulating immune response. To test its possible interaction with leptin within the immune system and beyond, we compared the transcription patterns of TNF, leptin and their cognate receptors obtained by meta-analysis of GenBank RNA-seq data. While expression of leptin and its receptor (LEPR) were detected in the brain and digestive tract, TNF and its receptor mRNAs were primarily found in viral-infected and LPS-treated leukocytes. We confirmed leptin expression in the duodenum by immunohistochemistry staining. Altogether, we suggest that whereas leptin and TNF interact as adipokines in mammals, in birds, they have distinct roles. Thus, the interaction between leptin and TNF may be unique to mammals.

Scientific Publication
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